MUC5AC (clone 45 M1), anti-human, mouse, rat, cat, chicken, hedgehog, monkey, pig, rabbit

Source:
A BALB/c mouse was immunized with mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) from an ALeb patient.
Fusion partner: SP2/0.

Specifications:
45M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the ‘h’ epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 45M1 and 2-12M1 both specifically react with epitopes located in the C-terminal cysteine rich part of the peptide core of MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Species reactivity:
Positive: cat, chicken, hedgehog, human, monkey, mouse, pig, rabbit, rat. Negative: cow.

Applications:
45M1 reacts with MUC5AC after citrate (/periodate) pretreatment. Immunoblotting: strongly positive without betha-mercaptoethanol pretreatment of mucin solution. ELISA or IRMA as component of a mixture of anti-M1 MAbs.
ELISA: +
Frozen sections: +
Paraffin sections: Citrate/periodate
Western blot: +

Positive control: Stomach, gastric cancer.

References:
- Bara, J. et al, Int. J. Cancer 47: 304-310 (1991).
- Bara, J. et al, J. Immunol. Methods149: 105-113 (1992).