myNAT™-1 Rapid Integrated Real-time PCR Panel Analyzer

Breakthrough Sample-to-Answer Open PCR Platform

The myNAT™ system by OnsiteGene Inc. is an innovative, all-in-one molecular diagnostics platform for fast, compact and high-sensitivity multiplex panel nucleic acid testing, with an open cartridge for users to make a test with their own regents capable of 1 sample throughput. ™All its reagents and test cartridges can be shipped and stored at room temperature for 12 months, ideal for PCT.

With an ultra-low-cost sealed cartridge design to achieve user flexibility of 1 to 4 PCR tubes (1-16/20 targets, and expandable to 8 PCR tubes for 40 target multiplexing), myNAT™ brings laboratory-grade performance into compact, point-of-need settings at significantly lower cost, particularly suitable for cost-sensitive applications.

The outstanding performance and innovative design of myNAT™ rapid nucleic acid panel testing system with a goal to reach sub-10minute PCR test have been recognized and selected for prestigious Advance Diagnostic Technology (RADx) program by the National Institute of Health (NIH).

Key Advantages

Compact & Portable (1 sample)
- Lightweight (7.6 kg), easy to deploy everywhere

Sample-to-Answer Automation free of contamination
- Integrated magnetic bead extraction + real-time PCR panel + melt curve analysis on iCard with contamination control

Flexible multiplex PCR panel testing
- 1-16/20 plex selectable over 4 PCR tubes in a panel, expandable to 40 plex

Cost-optimized Cartridge
- The novel sealed cartridge design dramatically reduces per-test costs while ensuring effective aerosol contamination control and supporting high-volume production-making it ideal for cost-sensitive applications

Scalable & Flexible
- Support random-access testing with multi-unit configurations (1-64 samples)Room temperature shipping and storage of test kits and cartridges
- Dried reagents are use din the test cartridges with shelf-life of 12 months

Compliant Manufacturing
- Produced in ISO-13485-audited facilities

Product Certification
- EN61010, EN61326

Versatile Sample Compatibility
- Swabs, saliva, sputum, blood, urine, feces, and tissue


Technical Specifications

Technology
- Sample-to-answer ultra-low-cost cartridge-based rapid nucleic acid analyzer with integrated magnetic bead extraction and a qPCR panel of up to 16 and optional 20 targets, capable of DNA melting curve analysis

Sample Throughput
- 1 sample (myNAT™-1)

Cartridge format
- Closed, preloaded with extraction/PCR reagents or user loaded

Sample-to-answer time
- 35 minutes

Sample Volume Range
- 100 µl – 500 µl

Panel layout
- 4 PCR tubes (up to16/20 targets), expandable to 40 targets, 10 µl – 50 µl reaction volume

Sensitivity (resolution)
- Target concentration changes as small as 2-fold difference can be detected in single plex fluorescence reactions

Dynamic range
- 8-fold dilution gradient

Optical channels
- Fam™, Hex™, Rox™, Cy5™. Option Cy5.5™

Heating Temperature
- 40-100 ° supports lysis and elution heating

Mixing Speed
- Adjustable with 6-speed modes

Precision
- CV<3% (based on 20 replicates at 2x LoD concentration, measured by Ct value

Sample type
- Swabs, blood, sputum, feces, urine, tissue, etc.

Operation
- Touch screen interface

User Interface
- Touch

Custom Protocol Support
- Yes (user-defined protocols)

Dimensions (W x D x H)
- 15 x 36 x 38 cm / 7.6 kg for myNAT™-1

Power Rating

- 100V – 240V AC, 50/60Hz


Software
The embedded touchscreen software provides a seamless, intuitive interface for efficient operation. Fully integrated into the instrument, it allows easy customization of both extraction and PCR protocols, and supports on-demand upgrades to keep your system up to date. For detailed features and step-by-step guidance, please consult the user manual.

Testing Kits

Broad Applications Powered by Sample-to-Answer Automation and Open-Panel Flexibility

The myNAT™ system is designed to support a wide range of molecular testing applications across healthcare, agriculture, environmental, and regulatory domains. Its fully automated sample-to-answer workflow—combining magnetic bead extraction and real-time multiplex PCR in a sealed cartridge—enables fast, accurate results with minimal hands-on time. The open-panel cartridge design allows users to customize targets and reagents, making it ideal for both standardized and lab-developed tests.

Key application areas include:
• Syndromic Infectious Disease Testing
- Rapidly detect multiple pathogens in a single test for respiratory, gastrointestinal, and other syndromic panels.
• Small, Medium, and Large Panel Testing
- Scale from single-plex to 40-plex testing across a variety of diseases or targets using flexible panel configurations.
• Environmental & Water Quality Testing
- Monitor microbial contamination and waterborne pathogens with fast, on-site molecular analysis.
• Food & Agricultural Safety
- Screen for GMOs, pathogens, and contaminants to ensure safety in food production and farming environments.
• Antimicrobial Resistance Screening
- Identify key resistance genes quickly to guide clinical or public health interventions.
• Life Sciences & Lab-Developed Tests (LDTs)
- Use the open cartridge format to develop and validate custom assays for research or specialized clinical needs.
• Veterinary & Pet Diagnostics
- Enable rapid diagnostics for animal health in both clinical and field settings.
• Customs, CDC, and Quarantine Testing
- Deploy fast, on-site screening for pathogens at ports of entry or during outbreak containment efforts.

With its room-temperature-stable cartridges, open platform versatility, and rapid 35-minute turnaround, myNAT™ empowers labs and field teams alike to bring high-performance molecular diagnostics anywhere they’re needed.