NADP Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181023
Application:
NADP covalently bound to Separopore® has a high affinity for NADP-dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP-Separopore® 4B-CL twelve times higher than NAD-Separopore® 4B-CL. NADP-Separopore® 4B-CL has also been used to purify extracellular nucleases.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: NADP
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size: 52 – 165 µm
Ligand density: Cyanogen bromide
Matrix attachment: N-6
Ligand density: 2 µmol NADP / ml drained gel
pH stability: 4 – 10
Supplied as lyophilized powder stabilized with lactose,
Swelling: 1 g swells to approx. 5 ml
References:
Bovine lens aldehyde reductase (aldose reductase). Purification, kinetics and mechanism. Biochem J. (1984) 219: 33-9.
Purification and structural properties of isozymes of isocitrate dehydrogenase from the mouse. Mol Cell Biochem. (1979) 23: 177-84.
12alpha-Hydroxysteroid dehydrogenase from Clostridium group P strain C48-50 ATCC No. 29733: partial purification and characterization. J Lipid Res. (1979) 20: 234-9.
Sulfate-mediated affinity chromatography on NADP+-Sepharose of glutamate dehydrogenase from halophilic bacteria and of glucose-6-phosphate dehydrogenase from Escherichia coli. Eur J Biochem. (1978) 84: 133-9.
Methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formyltetrahydrofolate synthetase from porcine liver. Isolation of a dehydrogenase-cyclohydrolase fragment from the multifunctional enzyme. Biochim Biophys Acta. (1977) 485: 52-9.
NADPH-cytochrome P-450 reductase from rat liver: purification by affinity chromatography and characterization. Biochemistry. (1977) 16: 1116-23.
An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase. Biochem J. (1977) 161: 561-8.
Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland. Biochem J. (1975) 151: 263-70.
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ATP-Separopore® 4B-CL (SKU # 20181051)
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NAD-Separopore® 4B-CL (Lyophilized) (SKU # 20181024)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: -20°C
Brand Name: bioPLUS™, Separopore®
NADP covalently bound to Separopore® has a high affinity for NADP-dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP-Separopore® 4B-CL twelve times higher than NAD-Separopore® 4B-CL. NADP-Separopore® 4B-CL has also been used to purify extracellular nucleases.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: NADP
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size: 52 – 165 µm
Ligand density: Cyanogen bromide
Matrix attachment: N-6
Ligand density: 2 µmol NADP / ml drained gel
pH stability: 4 – 10
Supplied as lyophilized powder stabilized with lactose,
Swelling: 1 g swells to approx. 5 ml
References:
Bovine lens aldehyde reductase (aldose reductase). Purification, kinetics and mechanism. Biochem J. (1984) 219: 33-9.
Purification and structural properties of isozymes of isocitrate dehydrogenase from the mouse. Mol Cell Biochem. (1979) 23: 177-84.
12alpha-Hydroxysteroid dehydrogenase from Clostridium group P strain C48-50 ATCC No. 29733: partial purification and characterization. J Lipid Res. (1979) 20: 234-9.
Sulfate-mediated affinity chromatography on NADP+-Sepharose of glutamate dehydrogenase from halophilic bacteria and of glucose-6-phosphate dehydrogenase from Escherichia coli. Eur J Biochem. (1978) 84: 133-9.
Methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formyltetrahydrofolate synthetase from porcine liver. Isolation of a dehydrogenase-cyclohydrolase fragment from the multifunctional enzyme. Biochim Biophys Acta. (1977) 485: 52-9.
NADPH-cytochrome P-450 reductase from rat liver: purification by affinity chromatography and characterization. Biochemistry. (1977) 16: 1116-23.
An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase. Biochem J. (1977) 161: 561-8.
Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland. Biochem J. (1975) 151: 263-70.
Related products:
ADP-Separopore® 4B-CL (Lyophilized) (SKU # 20181057)
AMP-Separopore® 4B-CL (Lyophilized) (SKU # 20181000)
ATP-Separopore® 4B-CL (Lyophilized) (SKU # 20181080)
ATP-Separopore® 4B-CL (SKU # 20181051)
N-Acetyl-galactosamine Separopore® 4B-CL (SKU # 20181021)
N-Acetyl-glucosamine Separopore® 4B-CL (SKU # 20181022)
GTP-Separopore® 4B-CL (SKU # 20181066)
NAD-Separopore® 4B-CL (Lyophilized) (SKU # 20181024)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: -20°C
Brand Name: bioPLUS™, Separopore®
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