Neurofilament (180kDa NF-H) (clone NE14), anti-human, mouse, rat, bovine, cat, chicken, gerbil, guinea pig, pig, rabbit

Source:
A BALB/c mouse was immunized with a crude neurofilament preparation from porcine spinal cord.

Specifications:
NE14 reacts specifically with the heavily phosphorylated KSP/KEP segment at the C-terminus of the 200 kDa subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase NE14 no longer binds. Neuronal intermediate filaments are typically referred to as a neurofilament triplet of low (L), middle (M) and high (H) molecular weight subunits of 68kDa, 160 kDa and 200 kDa, respectively. Like other anti-NF antibodies, NE-14. recognizes a phosphorylated epitope on a component of sarcomers of striated muscle, identified as myomesin, but also reported as the closely associated titin. MAbs raised to titin also cross react with NF.

Species reactivity:
Positive: cat, chicken, cow, gerbil, guinea pig, human, mouse, pig, rabbit, rat.

Applications:
NR4 can be applied for immunohistochemistry on frozen and paraffin sections and for Western blot analysis. Neuromas, gangliogliomas, neuroblastomas and medulloblastomas are positive.
Frozen sections: +
Paraffin sections: Citrate
Western blot: +

Positive control: Brain, neuroblastoma.

References:
- Debus E., et al, Differentiation25(2): 193-203 (1983).
- Ma D. et al, Neuroscience68(1): 135-149 (1995).