Neurofilament (68kDa NF-L) (clone NR4), anti-human, mouse, rat, bovine, cat, chicken, gerbil, pig

Source:
A BALB/c mouse was immunized with a crude neurofilament preparation from porcine spinal cord, containing the triplet polypeptides of 200, 145 and 68 kDa.

Specifications:
NR4 reacts specifically with the 68 kDa subunit (NF-L) present in all neurofilaments. Neuronal intermediate filaments are typically referred to as a neurofilament triplet of low (L), middle (M) and high (H) molecular weight subunits of 68kDa, 150 kDa and 200 kDa, respectively. Neurofilaments constitute the main structural elements of neuronal axons and dendrites.

Species reactivity:
Positive: cat, chicken, cow, gerbil, human, mouse, pig, rat.

Applications:
NR4 can be applied for immunohistochemistry on frozen and paraffin sections and for Western blot analysis. Neuromas, gangliogliomas, neuroblastomas and medulloblastomas are positive.
Frozen sections: +
Paraffin sections: Citrate
Western blot: +

Positive control: Brain, neuroblastoma.

References:
- Debus E, et al, Differentiation25(2): 193-203 (1983).
- Ma D. et al, Neuroscience68(1): 135-149 (1995)