NHS-Activated Separopore® 6B-CL (With Spacer Arm)
€0.00
In stock
SKU
BW-20181154
Application:
Rapid immobilization of antibodies and other proteins to the matrix.
Immobilization of ligands through primary amines to purify recombinant proteins.
Immobilization of Protein A or Protein G for monoclonal antibody purification.
Pre-activated medium for immobilization of ligands containing a primary amino group.
N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands.
The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
Coupling reaction can also be carried out in organic solvents.
Provides a 10 atom spacer arm upon ligand coupling.
Coupling completed after 2 – 4 h at room temperature.
NHS-Activated Separopore® provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: Primary -NH2
Matrix: Separopore® 6B-CL (highly crosslinked agarose beads, 4%)
Particle size range: 53 – 180 μm
Molecular weight range: 6 x 104 – 2 x 107
Chemical linkage of ligand: Amide bond
Spacer arm: 6-aminocaproic acid, 10 atoms
Substitution: >18 μmol NHS / ml drained gel
pH stability: 3 – 13 (ligand dependent)
Coupling conditions: pH 6 – 9; Temp: 4 – 25 °C
Storage: 2 – 8 °C
Flow rate Specifications: 70 – 140 cm / h
Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups
References:
Epitope structure of the carbohydrate recognition domain of asialoglycoprotein receptor to a monoclonal antibody revealed by high-resolution proteolytic excision mass spectrometry. J Am Soc Mass Spectrom. (2011) 22: 148-57.
Hepatitis B virus core interacts with the host cell nucleolar protein, nucleophosmin 1. J Microbiol. (2009) 47: 746-52.
Using liposomal fluorescent biolabels to develop an immunoaffinity chromatographic biosensing system for biotin. Anal Chem. (2008) 80: 6405-9.
Separation of protein C from Cohn Fraction IV-1 by mini-antibody. Adv Exp Med Biol. (2007) 599: 125-31.
Chromatographic refolding of recombinant human interferon gamma by an immobilized sht GroEL191-345 column. J Chromatogr A. (2006) 1107: 192-7.
Sephadex-based cell-affinity adsorbents: preparation and performance. Biotechnol Appl Biochem. (2002) 35: 55-60.
Purification and characterization of cytokine-inducing protein of seed extract from Aeginetia indica L., a parasitic plant. Immunopharmacology. (2000) 49: 377-89.
A method for the purification of cAMP-dependent protein kinase using immunoaffinity chromatography. Protein Expr Purif. (1998) 14: 418-24.
Peptide synthesis on Sepharose beads. J Pept Res. (1997) 49: 355-62.
Inhibition of Holliday structure resolving endonuclease VII of bacteriophage T4 by recombination enzymes UvsX and UvsY. J Mol Biol. (1997) 267: 150-62.
Construction of a bioreactor to produce special breakdown products of phytate. J Biotechnol. (1996) 48: 153-9.
Protein-protein interaction affinity chromatography of leukotriene C4 synthase. Protein Expr Purif. (1995) 6: 352-6.
Recombinant human insulin receptor substrate-1 protein. Tyrosine phosphorylation and in vitro binding of insulin receptor kinase. J Biol Chem. (1995) 270: 4870-4.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Rapid immobilization of antibodies and other proteins to the matrix.
Immobilization of ligands through primary amines to purify recombinant proteins.
Immobilization of Protein A or Protein G for monoclonal antibody purification.
Pre-activated medium for immobilization of ligands containing a primary amino group.
N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands.
The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
Coupling reaction can also be carried out in organic solvents.
Provides a 10 atom spacer arm upon ligand coupling.
Coupling completed after 2 – 4 h at room temperature.
NHS-Activated Separopore® provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: Primary -NH2
Matrix: Separopore® 6B-CL (highly crosslinked agarose beads, 4%)
Particle size range: 53 – 180 μm
Molecular weight range: 6 x 104 – 2 x 107
Chemical linkage of ligand: Amide bond
Spacer arm: 6-aminocaproic acid, 10 atoms
Substitution: >18 μmol NHS / ml drained gel
pH stability: 3 – 13 (ligand dependent)
Coupling conditions: pH 6 – 9; Temp: 4 – 25 °C
Storage: 2 – 8 °C
Flow rate Specifications: 70 – 140 cm / h
Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups
References:
Epitope structure of the carbohydrate recognition domain of asialoglycoprotein receptor to a monoclonal antibody revealed by high-resolution proteolytic excision mass spectrometry. J Am Soc Mass Spectrom. (2011) 22: 148-57.
Hepatitis B virus core interacts with the host cell nucleolar protein, nucleophosmin 1. J Microbiol. (2009) 47: 746-52.
Using liposomal fluorescent biolabels to develop an immunoaffinity chromatographic biosensing system for biotin. Anal Chem. (2008) 80: 6405-9.
Separation of protein C from Cohn Fraction IV-1 by mini-antibody. Adv Exp Med Biol. (2007) 599: 125-31.
Chromatographic refolding of recombinant human interferon gamma by an immobilized sht GroEL191-345 column. J Chromatogr A. (2006) 1107: 192-7.
Sephadex-based cell-affinity adsorbents: preparation and performance. Biotechnol Appl Biochem. (2002) 35: 55-60.
Purification and characterization of cytokine-inducing protein of seed extract from Aeginetia indica L., a parasitic plant. Immunopharmacology. (2000) 49: 377-89.
A method for the purification of cAMP-dependent protein kinase using immunoaffinity chromatography. Protein Expr Purif. (1998) 14: 418-24.
Peptide synthesis on Sepharose beads. J Pept Res. (1997) 49: 355-62.
Inhibition of Holliday structure resolving endonuclease VII of bacteriophage T4 by recombination enzymes UvsX and UvsY. J Mol Biol. (1997) 267: 150-62.
Construction of a bioreactor to produce special breakdown products of phytate. J Biotechnol. (1996) 48: 153-9.
Protein-protein interaction affinity chromatography of leukotriene C4 synthase. Protein Expr Purif. (1995) 6: 352-6.
Recombinant human insulin receptor substrate-1 protein. Tyrosine phosphorylation and in vitro binding of insulin receptor kinase. J Biol Chem. (1995) 270: 4870-4.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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