Ni-IMAC Separopore® 4B

Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Imminodiacetate immobilized matrix charged with Ni metal ions.
Chelating group coupling method: epoxy activation
Matrix: cross-linked Separopore® 6B
Particle size (range): 50-170µm
Metal ion capacity: > 20µmol Ni2+/ml gel
Spacer arm: 14 atom
pH stability: 3-12
Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
Supplied as suspension in 20% ethanol.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®