Nickel Red Separopore® 4B Microbeads viewIP™
€0.00
In stock
SKU
BW-20181117
Application:
Affinity matrix that is red colored, immobilized metal-ion affinity chromatography (IMAC) designed for use in small scale affinity capture (molecular pull-down) experiments. Red IMAC gel consists of quadridentrate chelate bound with nickel and covalently attached to Red beaded 6% agarose (Separopore® 6B). View-IP™ His-tag Nickel Red-Separopore® 6B-CL is convenient for small-scale affinity capture of fusion proteins with histidine containing tags and greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Nitrilotriacetic acid (NTA)
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size (range): 52 – 165 µm
Matrix activation: Cyanogen bromide
Binding capacity: approximately 15 mg His-tagged protein/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS
Storage: 2 – 8 °
References:
Overexpression of potassium channel ether à go-go in human osteosarcoma. Neoplasma. (2012) 59: 207-15.
Cloning and expression of a thermostable alpha-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris. J Microbiol Biotechnol. (2010) 20: 1653-63.
Antitumour response of a double mutant of staphylococcal enterotoxin C2 with the decreased affinity for MHC class II molecule. Scand J Immunol. (2010) 71: 169-75.
Polymorphic membrane proteins 1 and 7 from Chlamydophila felis are significant immunodominant proteins. Vet Microbiol. (2010) 144: 415-21.
Development of a bacteriophage-based system for the selection of structured peptides. Anal Biochem. (2009) 388: 122-7.
WNT5A is regulated by PAX2 and may be involved in blastemal predominant Wilms tumorigenesis. Neoplasia. (2008) 10: 1470-80.
Prokaryotic expression of truncated VP7 of bluetongue virus (BTV) and reactivity of the purified recombinant protein with all BTV type-specific sera. J Virol Methods. (2008) 152: 6-12.
Prophylactic evaluation of recombinant extracellular superoxide dismutase of Brugia malayi in jird model. Vaccine. (2008) 26: 3705-10.
A novel method to capture methylated human DNA from stool: implications for colorectal cancer screening. Clin Chem. (2007) 53: 1646-51.
The expression and purification of the N-terminal activation domain of the transcription factor c-Myc: a model substrate for exploring ERK2 docking interactions. Protein Expr Purif. (2007) 53: 80-6.
Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1. Folia Microbiol (Praha). (2006) 51: 492-6.
FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. Hum Mol Genet. (2006) 15: 3229-40.
Cloning of a gene fragment encoding bovine complement component C3d with expression and characterization of derived fusion proteins. Vet Immunol Immunopathol. (2006) 114: 61-71.
Production of recombinant Bartonella henselae 17-kDa protein for antibody-capture enzyme-linked immunosorbent assay. Diagn Microbiol Infect Dis. (2006) 55: 1-7.
Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex. Protein Expr Purif. (2006) 47: 264-72.
Analysis of phosphatase and tensin homolog tumor suppressor interacting proteins by in vitro and in silico proteomics. Proteomics. (2005) 5: 1250-62.
Identification of target genes regulated by FOXC1 using nickel agarose-based chromatin enrichment. Invest Ophthalmol Vis Sci. (2004) 45: 3904-13.
Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase. Protein Expr Purif. (2004) 37: 392-8.
Prevention of aggregation after refolding by balanced stabilization-destabilization: production of the Arabidopsis thaliana protein APG8a (At4g21980) for NMR structure determination. Protein Expr Purif. (2004) 34: 280-3.
Expression and purification of turkey coronavirus nucleocapsid protein in Escherichia coli. J Virol Methods. (2004) 116: 161-7.
Heat elution chromatography of immunoglobulins. Protein Expr Purif. (2003) 30: 301-3.
Trichomonas vaginalis Hmp35, a putative pore-forming hydrogenosomal membrane protein, can form a complex in yeast mitochondria. J Biol Chem. (2003) 278: 30548-61.
Recombinant (2-->3)-alpha-sialyltransferase immobilized on nickel-agarose for preparative synthesis of sialyl Lewis(x) and Lewis(a) precursor oligosaccharides. Carbohydr Res. (2003) 338: 1153-61.
Expression and purification of a recombinant form of human aromatase from Escherichia coli. Biochem Pharmacol. (2002) 64: 1317-24.
Overexpression of Arabidopsis thaliana soluble epoxide hydrolase 1 in Pichia pastoris and characterisation of the recombinant enzyme. Protein Expr Purif. (2002) 26: 65-70.
Combined biosynthetic pathway for de novo production of UDP-galactose: catalysis with multiple enzymes immobilized on agarose beads. Chembiochem. (2002) 3: 348-55.
Low concentrations of free hydrophobic amino acids disrupt the Escherichia coli RNA polymerase core-sigma(70) protein-protein interaction. Protein Expr Purif. (2002) 24: 163-70.
Correction of cross-linker sensitivity of Fanconi anemia group F cells by CD33-mediated protein transfer. Blood. (2001) 98: 3817-22.
Purification of a model substrate for transcription factor phosphorylation by ERK2. Protein Expr Purif. (2001) 23: 191-7.
Diagnostic immunoassays for tick-borne encephalitis virus based on recombinant baculovirus protein expression. J Virol Methods. (2001) 91: 75-84.
Expression and purification of recombinant human indoleamine 2, 3-dioxygenase. Protein Expr Purif. (2000) 19: 22-9.
Selenoprotein P expression, purification, and immunochemical characterization. J Biol Chem. (2000) 275: 6288-94.
Expression and functional interaction of the catalytic and regulatory subunits of human methionine adenosyltransferase in mammalian cells. J Biol Chem. (1999) 274: 29720-5.
Functional interactions between conserved motifs of the hepatitis C virus RNA helicase protein NS3. Virus Genes. (1999) 19: 33-43.
The U69 gene of human herpesvirus 6 encodes a protein kinase which can confer ganciclovir sensitivity to baculoviruses. J Virol. (1999) 73: 3284-91.
Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. J Bacteriol. (1999) 181: 718-25.
Cooperativity between two heads of dictyostelium myosin II in in vitro motility and ATP hydrolysis. Biophys J. (1999) 76: 985-92.
Antitumor response elicited by a superantigen-transmembrane sequence fusion protein anchored onto tumor cells. J Immunol. (1998) 161: 6761-7.
Gene structure, expression and chromosomal localization of murine theta class glutathione transferase mGSTT1-1. Biochem J. (1999) 337: 141-51.
Interactions of phosducin with the subunits of G-proteins. Binding to the alpha as well as the betagamma subunits. J Biol Chem. (1998) 273: 9465-71.
Detection of IgG autoantibodies in the sera of patients with bullous and gestational pemphigoid: ELISA studies utilizing a baculovirus-encoded form of bullous pemphigoid antigen 2. J Invest Dermatol. (1998) 110: 282-6.
A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice. Eur J Immunol. (1997) 27: 3447-55.
Evidence that human class Theta glutathione S-transferase T1-1 can catalyse the activation of dichloromethane, a liver and lung carcinogen in the mouse. Comparison of the tissue distribution of GST T1-1 with that of classes Alpha, Mu and Pi GST in human. Biochem J. (1997) 326: 837-46.
Dynamic interplay of TFIIA, TBP and TATA DNA. J Mol Biol. (1997) 271: 61-75.
Cloning of cDNA encoding rat spermatidal protein TP2 and expression in Escherichia coli. Protein Expr Purif. (1996) 8: 409-15.
Expression and purification of recombinant toxicshock-syndrome toxin I. Biotechnol Appl Biochem. (1996) 24: 155-9.
A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding. Biochemistry. (1996) 35: 4540-50.
Expression of a recombinant derivative of human aromatase P450 in insect cells utilizing the baculovirus vector system. Mol Cell Endocrinol. (1995) 109: R1-5.
High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J Virol. (1994) 68: 766-75.
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®, ViewIP™
Affinity matrix that is red colored, immobilized metal-ion affinity chromatography (IMAC) designed for use in small scale affinity capture (molecular pull-down) experiments. Red IMAC gel consists of quadridentrate chelate bound with nickel and covalently attached to Red beaded 6% agarose (Separopore® 6B). View-IP™ His-tag Nickel Red-Separopore® 6B-CL is convenient for small-scale affinity capture of fusion proteins with histidine containing tags and greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Nitrilotriacetic acid (NTA)
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size (range): 52 – 165 µm
Matrix activation: Cyanogen bromide
Binding capacity: approximately 15 mg His-tagged protein/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS
Storage: 2 – 8 °
References:
Overexpression of potassium channel ether à go-go in human osteosarcoma. Neoplasma. (2012) 59: 207-15.
Cloning and expression of a thermostable alpha-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris. J Microbiol Biotechnol. (2010) 20: 1653-63.
Antitumour response of a double mutant of staphylococcal enterotoxin C2 with the decreased affinity for MHC class II molecule. Scand J Immunol. (2010) 71: 169-75.
Polymorphic membrane proteins 1 and 7 from Chlamydophila felis are significant immunodominant proteins. Vet Microbiol. (2010) 144: 415-21.
Development of a bacteriophage-based system for the selection of structured peptides. Anal Biochem. (2009) 388: 122-7.
WNT5A is regulated by PAX2 and may be involved in blastemal predominant Wilms tumorigenesis. Neoplasia. (2008) 10: 1470-80.
Prokaryotic expression of truncated VP7 of bluetongue virus (BTV) and reactivity of the purified recombinant protein with all BTV type-specific sera. J Virol Methods. (2008) 152: 6-12.
Prophylactic evaluation of recombinant extracellular superoxide dismutase of Brugia malayi in jird model. Vaccine. (2008) 26: 3705-10.
A novel method to capture methylated human DNA from stool: implications for colorectal cancer screening. Clin Chem. (2007) 53: 1646-51.
The expression and purification of the N-terminal activation domain of the transcription factor c-Myc: a model substrate for exploring ERK2 docking interactions. Protein Expr Purif. (2007) 53: 80-6.
Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1. Folia Microbiol (Praha). (2006) 51: 492-6.
FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. Hum Mol Genet. (2006) 15: 3229-40.
Cloning of a gene fragment encoding bovine complement component C3d with expression and characterization of derived fusion proteins. Vet Immunol Immunopathol. (2006) 114: 61-71.
Production of recombinant Bartonella henselae 17-kDa protein for antibody-capture enzyme-linked immunosorbent assay. Diagn Microbiol Infect Dis. (2006) 55: 1-7.
Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex. Protein Expr Purif. (2006) 47: 264-72.
Analysis of phosphatase and tensin homolog tumor suppressor interacting proteins by in vitro and in silico proteomics. Proteomics. (2005) 5: 1250-62.
Identification of target genes regulated by FOXC1 using nickel agarose-based chromatin enrichment. Invest Ophthalmol Vis Sci. (2004) 45: 3904-13.
Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase. Protein Expr Purif. (2004) 37: 392-8.
Prevention of aggregation after refolding by balanced stabilization-destabilization: production of the Arabidopsis thaliana protein APG8a (At4g21980) for NMR structure determination. Protein Expr Purif. (2004) 34: 280-3.
Expression and purification of turkey coronavirus nucleocapsid protein in Escherichia coli. J Virol Methods. (2004) 116: 161-7.
Heat elution chromatography of immunoglobulins. Protein Expr Purif. (2003) 30: 301-3.
Trichomonas vaginalis Hmp35, a putative pore-forming hydrogenosomal membrane protein, can form a complex in yeast mitochondria. J Biol Chem. (2003) 278: 30548-61.
Recombinant (2-->3)-alpha-sialyltransferase immobilized on nickel-agarose for preparative synthesis of sialyl Lewis(x) and Lewis(a) precursor oligosaccharides. Carbohydr Res. (2003) 338: 1153-61.
Expression and purification of a recombinant form of human aromatase from Escherichia coli. Biochem Pharmacol. (2002) 64: 1317-24.
Overexpression of Arabidopsis thaliana soluble epoxide hydrolase 1 in Pichia pastoris and characterisation of the recombinant enzyme. Protein Expr Purif. (2002) 26: 65-70.
Combined biosynthetic pathway for de novo production of UDP-galactose: catalysis with multiple enzymes immobilized on agarose beads. Chembiochem. (2002) 3: 348-55.
Low concentrations of free hydrophobic amino acids disrupt the Escherichia coli RNA polymerase core-sigma(70) protein-protein interaction. Protein Expr Purif. (2002) 24: 163-70.
Correction of cross-linker sensitivity of Fanconi anemia group F cells by CD33-mediated protein transfer. Blood. (2001) 98: 3817-22.
Purification of a model substrate for transcription factor phosphorylation by ERK2. Protein Expr Purif. (2001) 23: 191-7.
Diagnostic immunoassays for tick-borne encephalitis virus based on recombinant baculovirus protein expression. J Virol Methods. (2001) 91: 75-84.
Expression and purification of recombinant human indoleamine 2, 3-dioxygenase. Protein Expr Purif. (2000) 19: 22-9.
Selenoprotein P expression, purification, and immunochemical characterization. J Biol Chem. (2000) 275: 6288-94.
Expression and functional interaction of the catalytic and regulatory subunits of human methionine adenosyltransferase in mammalian cells. J Biol Chem. (1999) 274: 29720-5.
Functional interactions between conserved motifs of the hepatitis C virus RNA helicase protein NS3. Virus Genes. (1999) 19: 33-43.
The U69 gene of human herpesvirus 6 encodes a protein kinase which can confer ganciclovir sensitivity to baculoviruses. J Virol. (1999) 73: 3284-91.
Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. J Bacteriol. (1999) 181: 718-25.
Cooperativity between two heads of dictyostelium myosin II in in vitro motility and ATP hydrolysis. Biophys J. (1999) 76: 985-92.
Antitumor response elicited by a superantigen-transmembrane sequence fusion protein anchored onto tumor cells. J Immunol. (1998) 161: 6761-7.
Gene structure, expression and chromosomal localization of murine theta class glutathione transferase mGSTT1-1. Biochem J. (1999) 337: 141-51.
Interactions of phosducin with the subunits of G-proteins. Binding to the alpha as well as the betagamma subunits. J Biol Chem. (1998) 273: 9465-71.
Detection of IgG autoantibodies in the sera of patients with bullous and gestational pemphigoid: ELISA studies utilizing a baculovirus-encoded form of bullous pemphigoid antigen 2. J Invest Dermatol. (1998) 110: 282-6.
A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice. Eur J Immunol. (1997) 27: 3447-55.
Evidence that human class Theta glutathione S-transferase T1-1 can catalyse the activation of dichloromethane, a liver and lung carcinogen in the mouse. Comparison of the tissue distribution of GST T1-1 with that of classes Alpha, Mu and Pi GST in human. Biochem J. (1997) 326: 837-46.
Dynamic interplay of TFIIA, TBP and TATA DNA. J Mol Biol. (1997) 271: 61-75.
Cloning of cDNA encoding rat spermatidal protein TP2 and expression in Escherichia coli. Protein Expr Purif. (1996) 8: 409-15.
Expression and purification of recombinant toxicshock-syndrome toxin I. Biotechnol Appl Biochem. (1996) 24: 155-9.
A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding. Biochemistry. (1996) 35: 4540-50.
Expression of a recombinant derivative of human aromatase P450 in insect cells utilizing the baculovirus vector system. Mol Cell Endocrinol. (1995) 109: R1-5.
High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J Virol. (1994) 68: 766-75.
Related products:
View-IP™ Protein A Red-Separopore® 4B-CL (SKU # 20181041)
View-IP™ Protein A Green-Separopore® 4B-CL (SKU # 20181108)
View-IP™ Protein A Blue-Separopore® 4B-CL (SKU # 20181105)
View-IP™ Protein G Red-Separopore® 4B-CL (SKU # 20181118)
View-IP™ Streptavidin Red-Separopore® 4B-CL (SKU # 20181103)
View-IP™ Streptavidin Green-Separopore® 4B-CL (SKU # 20181109)
View-IP™ Streptavidin Blue-Separopore® 4B-CL (SKU # 20181106)
View-IP™ Glutathione Red-Separopore® 4B-CL (SKU # 20181116)
View-IP™ Calmodulin Red-Separopore® 4B-CL (SKU # 20181104)
View-IP™ Calmodulin Green-Separopore® 4B-CL (SKU # 20181110)
View-IP™ Calmodulin Blue-Separopore® 4B-CL (SKU # 20181107)
View-IP™ Anti-HA Red-Separopore® 4B-CL (SKU # 20181115)
View-IP™ Anti-DYKDDDDK (FLAG) Red-Separopore® 4B-CL (SKU # 20181114)
View-IP™ Anti-cMyc Red-Separopore® 4B-CL (SKU # 20181113)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®, ViewIP™
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