Nucleoli (clone AE-3), anti-human

Source:
A BALB/c mouse was immunized with nuclei of myeloid leukemia biopsy cells.
Fusion partner: NS-1.

Specifications:
AE-3 recognizes a nucleolar antigen expressed in nucleoli of human cells. AE-3 can also be used as a marker of the nucleolus in subcellular fractions.

Species reactivity:
Positive: human.

Applications:
AE-3 produces a diffuse staining pattern of the nucleolus of normal and malignant cells and can be used for paraformaldehyde fixed or frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Flow cytometry: -
Frozen sections: +
Immunofluorescence: +
Paraffin sections: +

Positive control: Human tonsil.

References:
- Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In: Progress on non-histone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.