oCelloScope-Fungal Monitoring and Analysis

Real-time monitoring and analyses of your fungi

oCelloScope is an automated live-cell imaging system that speeds up microbiology research and gives a deeper understanding of the biological processes.
Using standard microtiter plates, you can analyse up to 96 microbial samples at a time, and perform real-time analysis inside the incubator, saving hours of valuable time while maintaining the quality of your experiments.

See movie oCelloScope Live Cell imaging - Aspergillus niger growth here

Get a deeper insight
• Reliable analyses of fungal growth
• Analyse morphogenesis in real-time
• Capture and quantify spores germination
• Visualise and validate the results with images and videos

Analyse any sample
• Supports analyses of a variety of microorganisms: Fungi, Yeast, Mold and Bacteria
• Analyse Mixed-cultures and complex samples
• Perform the analyses inside an incubator

Save time and money
• Never miss a data point again with continuous monitoring
• Set up, walk away, and get automated real-time analyses of your culture
• Use standard microtiter plates

Automated monitoring and analysis of fungal growth
Automated live monitoring and analysis of fungal growth is now possible with oCelloScope. OD is unreliable in measuring fungal growth, due to the morphology of the fungi and its non-uniform distribution. oCelloScope solves this issue by combining high resolution images with automated image analysis.
The SESA-Fungi algorithm, identifies the fungi in the image and quantifies the growth from spores to hyphae. Aunsbjerg SD et al. (2015), have shown that with oCelloScope results are obtained faster, compared to OD, resulting in a shorter incubation time.

See movie oCelloScope Live Cell imaging - Growth of Penicillum roquefortii here

Perform single cell analysis and follow the morphogenesis of your hyphae
With the build-in segmentation algorithms, the UniExplorer software enables the user to automatically perform single cell analysis, allowing scientists to follow the morphogenesis of single fungi cells, and discriminate and characterize the dynamics of different cell types in a complex sample.
More than 20 morphological features, including cell size and shape factors of each single object can be calculated and visualized in scatter plots and histograms, enabling the user to group the desired objects and analyse the morphology changes over time of the desired group.

P.roqueforti spore germination time-lapse.

As an example, discrimination between vegetative cells and spores in fungi or bacterial sporulation experiments can be performed.

Segmentation of a complex sample composed of yeast cells and 1µm beads.

Fungal Spore Tracking – Individual spores, each with its own ID tag followed over 31 time points.

By using the oCelloScope we were able to follow the germination of thousends of individual fungal conidiospores within a population or community. We could track developement of these individuals by tracing size and shape and determine the influence of different conditions on their outgrow. Thas as never done. Dr. Wieke R. Teertstra Researcher, Kruyt Gebouw, Utrecht University

Plate and biochemical assays can show strain and hit performances, but seeeing is believing. The High Througput Assay Development Team at Gingko Bioworks has incorporated the oCelloScope as an essential tool for validating and troubleshooting many of our assays. The oCelloScope reliably produces high-quality microscopy data from plate screens, building the scale of Gingko's platform to engineer living cells to generte better products. Customer and inter-team understanding of strain growth, treatments, and communication of assay conditions and performances is made easy with the oCelloScope.

Publications
2021
Antifungal Resistance in Isolates of Aspergillus from a Pig Farm
White, J, K., Nielsen, J, L., Poulsen, J, S., Madsen, A, M. (2021)MDPI – Atmosphere 12, 2021. Read more https://www.mdpi.com/2073-4433/12/7/826/htm

Inhibitory effects of binary combinations of microbial metabolites on the growth of tolerant Penicillium roqueforti and Mucor circinelloides
Shi, C., Knøchel, S. (2021)
LWT – Food Science and Technology 149, 2021. Read more

Physiological responses of Aspergillus niger challenged with itraconazole
Poulsen, J.S., Madsen A.M., White, J.K., Nielsen, J.L. (2021)
Antimicrobial Agents and Chemotherapy, April, 2021.Read more