Omni KlenTaq 2
€0.00
In stock
SKU
VN1900E
Catalog Number: VN1900E
Size: 125 µl (500~1000 x 25 µl rxns)
Other Size(s): 50 µl (200~400 x 25 µl rxns)
Datasheet
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Size: 125 µl (500~1000 x 25 µl rxns)
Other Size(s): 50 µl (200~400 x 25 µl rxns)
Datasheet
Request Information
Inhibition-resistant Taq Pols & Master Mixes
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Omni KlenTaq 2 is a new mutant of Klentaq DNA polymerase that makes the enzyme resistant to the inhibitory effects of higher concentrations of blood, soil, plant and more. It typically remains functional in 40% whole blood in PCR, and in some concentrations of crude soil extracts where other commercial enzymes fail. It is able to amplify the gene target directly from whole blood, serum, plasma, water, milk, tissue and plant leaf, etc. without DNA purification prior to PCR. This enzyme can be used in real-time qPCR with DNA binding dyes, such as SYBR Green and Eva Green, however, it can not be used in real-time qPCR TaqMan assay which requires 5’-3’ exonuclease activity.
Omni KlenTaq 2 LA version (Long & Accurate) is available, which allows for amplification of longer products with higher fidelity and accuracy.
Omni KlenTaq 2 & Omni KlenTaq 2 LA Mix are available as a ready-to-use 2X mixture of DNA polymerase, salts, magnesium and dNTPs for setting up a trouble-free PCR reaction.
Hot-start Omni KlenTaq 2 is a mixture of Omni KlenTaq 2 and an Aptamer-based inhibitor, which increase PCR specificity and sensitivity (Click here).
- Thermostability – more thermostable than standard Taq DNA polymerase- Efficient – novel buffer system maximizes efficiency of PCR amplification, increases yield of any PCR product- Robust and Inhibition-resistant – reliable direct amplification from crude samples and with even the most challenging DNA targets, especially when combining with the PCR enhancers- Flexible – LA version is ideal for amplifying any target up to 30 kb from DNA extracted from human, animal and plant samples
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Omni KlenTaq 2 is a new mutant of Klentaq DNA polymerase that makes the enzyme resistant to the inhibitory effects of higher concentrations of blood, soil, plant and more. It typically remains functional in 40% whole blood in PCR, and in some concentrations of crude soil extracts where other commercial enzymes fail. It is able to amplify the gene target directly from whole blood, serum, plasma, water, milk, tissue and plant leaf, etc. without DNA purification prior to PCR. This enzyme can be used in real-time qPCR with DNA binding dyes, such as SYBR Green and Eva Green, however, it can not be used in real-time qPCR TaqMan assay which requires 5’-3’ exonuclease activity.
Omni KlenTaq 2 LA version (Long & Accurate) is available, which allows for amplification of longer products with higher fidelity and accuracy.
Omni KlenTaq 2 & Omni KlenTaq 2 LA Mix are available as a ready-to-use 2X mixture of DNA polymerase, salts, magnesium and dNTPs for setting up a trouble-free PCR reaction.
Hot-start Omni KlenTaq 2 is a mixture of Omni KlenTaq 2 and an Aptamer-based inhibitor, which increase PCR specificity and sensitivity (Click here).
- Thermostability – more thermostable than standard Taq DNA polymerase- Efficient – novel buffer system maximizes efficiency of PCR amplification, increases yield of any PCR product- Robust and Inhibition-resistant – reliable direct amplification from crude samples and with even the most challenging DNA targets, especially when combining with the PCR enhancers- Flexible – LA version is ideal for amplifying any target up to 30 kb from DNA extracted from human, animal and plant samples
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