One-Step RT PCR Kit

One-Step RT PCR Kit is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube. The kit includes a PCR MasterMix supplied in a 2x concentration to perform standard PCR.

MasterMix contains all the reagents needed for running PCR reactions (except PCR primers and template). In addition, a separate RT Mix composed by a balanced mixture of both RTase and RNase Inhibitor is also provided.

RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA Polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA Polymerase exponentially amplifies the double-stranded DNA template.

Advantages & Features
- Higher specificity, sensitivity, and yield.
- Efficient: thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields.
- Unique: Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs.
- Highly Sensitive: PCR enhancers allowing sensitive low background amplification.

Includes
- 2 x 1.25 mL One-Step PCR Mix (2x)
- 2 x 125 μL RT Mix
- 2 mL Water (RNase-free)

Applications
- One-step RT-PCR.
- Amplification of GC-rich and complex templates.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C (NON Frost-Free Freezer).