p21/WAF1 (clone WA-1), anti-human, mouse, rat, chimpanzee, monkey

Source:
A BALB/c mouse was immunized with human p21 protein.
Fusion partner: Sp2/0.

Specifications:
WA-1 reacts with human and other mammalian p21, a tumor suppressor protein, belonging to the CDI family. The intracellular protein p21 is a 21 kDa protein, also known as wild-typep53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and of proliferating-cell nuclear antigen (PCNA). It is induced by wild type p53, but not by mutated p53, by mezerein (anti-leukemic compound) and by interferon-ß. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma and thyroid carcinoma).

Species reactivity:
Positive: human, chimpanzee, monkey, mouse, rat.

Applications:
WA-1 can be used in immunohistochemistry, immunofluorescence tests and ELISA, both as solid phase and as tracer antibody.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: MCF7 cells, UV treated fibroblasts, HeLa cells, skin, colon, or breast carcinoma.

References:
- Kovaric, J. et al, Int. J. Oncol.9(suppl.), 835 (1996)