p47-phox (phospho-S304) polyclonal, anti-human, mouse, rat

p47-phox (phospho-S304) polyclonal, anti-human, mouse, rat

€328.00
In stock
SKU
BS4600
Catalog Number: BS4600
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: IHC

Datasheet
Background:
The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Alternative Name:
Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A

Application Dilution: IHC: 1:50~1:200

Specificity: p-p47-phox (S304) polyclonal antibody detects endogenous levels of p47-phox protein only when phosphorylated at Ser304.

Immunogen:
Synthetic phosphopeptide derived from human p47-phox around the phosphorylation site of Serine 304.

MW: ~ 45 kDa

Swis Prot.: P14598

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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