PDQeX Nucleic Acid Extractor
Catalog Number: XMA
- MicroGEM’s automated solution for effortless DNA extraction.
- Ideal for PCR, qPCR, STR, and Whole Genome Sequencing.
- Optimized for lysis and purification for double-stranded sequencing; also produces high-quality single-stranded extractions.
- Simplified workflow in a single closed-tube system to handle challenging samples such as plant and fungi.
- Perfect for labs looking for fast, hands-free, programmable sample processing.
- Produces DNA in less than 20 minutes
Price on request. Contact us
MicroGEM’s PDQeX Nucleic Acid Extractor is the next generation of thermocyclers. It uses powerful enzyme-driven extraction reagents with an innovative extractor cartridge and purification matrix. Just add heat and the closed-tube system produces DNA extracts in significantly less time (minutes rather than hours) than other extraction methods.
- PDQeX Extraction Reagents consist of a cocktail of thermophilic proteinase and mesophilic cell wall degrading enzymes that systematically lyse cells, destroy nucleases, digest proteins and release nucleic acids
- PDQeX Extractor Cartridges, made from thermo-responsive polymers, pump extracted DNA through the purification medium while removing inhibitors
- PDQeX Temperature-Controlled Extraction activates the proteinase and forces the extract through a heat-burstable valve and proprietary purification matrix, removing cell debris and inhibitors.
Resources:
PDQeX Product Manual (pdf)
PDQeX Quick-Start Guide prepGEM Bacteria (pdf)
PDQeX Quick-Start Guide forensicGEM Sperm (pdf)
PDQeX Extraction Cartridge Video (watch it here)
MicroGEM Brochure (watch it here)
MicroGEM Webinar (watch it here)
Literature:
Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX. BioTechniques. Vol.66, No. 5. 2019 (pdf)
This study compared a conventional column purification method with the PDQeX Nucleic Acid Extractor for purifying DNA for whole genome sequencing. Based on six bacterial suspensions, the PDQeX performed much more rapidly – in under 30 minutes – while producing comparable high-quality extractions.
Shaffer, L. Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences,116(9), 3351-3353. doi:10.1073/pnas. 1901806116. 2019 (pdf)
The article describes the ability to screen Cassava crops for Cassava Mosaic Virus (CMV) using MicroGEM’s PDQeX for rapid DNA extraction. The PDQeX was used as the enabling technology to complement DNA sequencing being conducted in the field in real-time.
PDQeX Accesories
| PDQeX Nucleic Acid Extractor | phytoGEM Starter Kit | phytoCards |
| Crusher Tool | Punch Tool and Tip | Punch Tool Replacement |
Specifications
| Sample Capacity | 24 samples, standard 96 well spacing |
| For use with | PDQeX cartridges (200 µL) |
| Interface | 5″ touch screen (colour) |
| Voltage (24 V DC adapter included) | 100-240 VAC, 2A, 50/60Hz |
| Power | 96 watts at peak draw |
| Thermal profiles | Pre-installed & user programmable |
| Programmable thermal range | 34-120 |
| Dimensions | 22 x 18 x 18.5 cm (L x W x H) |
| Weight | 4.8 kg |
| Is Featured? | No |
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