Phospho-Serine, -Threonine, & -Tyrosine Immunocytochemistry Kit

Background:
Phosphorylation of specific tyrosine, serine, and threonine residues is an important post-translational modification for regulating the activity of proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine, phosphoserine, or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phospho-proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphorylation using anti-phosphotyrosine or anti-phosphoserine/threonine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. Immunocytochemistry can also be used to examine changes in the level and localization of phosphotyrosine, phosphoserine, or phosphothreonine in cells after drug stimulation or during specific cell states.

Buffer/Storage:
Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.