Phospho-Tyrosine, Serine, Threonine Antibody Sampler Kit

Background:
Phosphorylation of specific tyrosine (tyr), serine (ser), or threonine (thr) residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr and tyr kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine, phosphoserine, or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest, followed by detection of tyr, ser, and thr phosphorylation using these antibodies is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

Buffer/Storage:
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.