PickMutant™ Site-directed Mutagenesis Kit

PickMutant™ is a reliable, robust and highly efficient PCR-based Mutagenesis Kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol.

All these mutation could be obtained by PCR using a FastPANGEA™ High Fidelity DNA Polymerase and well-designed Mutagenesis primers. The assembled mutagenic PCR fragments is cloned into Canvax™ pSpark® Cloning vector, specially designed to clone blunt PCR fragments with high efficiency or into other vector designing, in this case, an additional specific vector primer pair.

Advantages & Features
- Highly Effective point mutations (single or multiple), deletions or insertions.
- Easy and fast protocol: it takes less than 3 hours in one step procedure.
- Cost avoidance: compatible with any bacterial strains or primers.
- Versatile: compatible with any Cloning vector.
- Efficient: includes highly efficient pSpark® to clone blunted fragments.
- Robust: simultaneous assemble and clone of PCR fragments.

Includes
- 150 μL Proofreading DNA Polymerase MasterMix (2x)
- 300 U Glue Enzyme (10 U/μL)
- 40 μL Glue Enzyme Buffer (10x)
- 5 μL Insert Control DNA
- 15 μL pSpark® I (20 ng/μL)

Applications
- Site-directed Mutagenesis.
- Study protein function.
- Identify enzyme active sites.
- Design new proteins.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).