Pig APOE (Apolipoprotein E) ELISA Kit
€0.00
In stock
SKU
ELK5994
Catalog Number: ELK5994
Reactivity: Pig
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.144 ng/mL
Information
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Reactivity: Pig
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.144 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E(APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E(APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E(APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E(APOE) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Metabolic pathway, Cardiovascular biology, Hepatology
Alternative Names:
Apo-E, AD2, Apoprotein, Alzheimer Disease 2(E4-Associated,Late Onset
Sample Type:
Serum, plasma and other biological fluids
Assay Type: Sandwich
Standard: 20 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E(APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E(APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E(APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E(APOE) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Metabolic pathway, Cardiovascular biology, Hepatology
Alternative Names:
Apo-E, AD2, Apoprotein, Alzheimer Disease 2(E4-Associated,Late Onset
Sample Type:
Serum, plasma and other biological fluids
Assay Type: Sandwich
Standard: 20 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
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