Pig ASD (Androstenedione) ELISA Kit
€0.00
In stock
SKU
ELK8707
Catalog Number: ELK8707
Reactivity: Pig
Standard Range: 156.25-10000 pg/mL
Sensitivity: 48.8 pg/mL
Information
Request Manual
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Reactivity: Pig
Standard Range: 156.25-10000 pg/mL
Sensitivity: 48.8 pg/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androstenedione(ASD) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Androstenedione(ASD). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androstenedione(ASD) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Endocrinology, Reproductive science, Hormone metabolism
Alternative Names:
4-Androstenedione, 4-Androstene-3,17-Dione
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Competitive Inhibition
Standard: 10000 pg/mL
Assay Length: 2h
Assay Length: For Research Use Only
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androstenedione(ASD) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Androstenedione(ASD). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androstenedione(ASD) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Endocrinology, Reproductive science, Hormone metabolism
Alternative Names:
4-Androstenedione, 4-Androstene-3,17-Dione
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Competitive Inhibition
Standard: 10000 pg/mL
Assay Length: 2h
Assay Length: For Research Use Only
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