Pig ASMA (Anti-Smooth Muscle Antibody) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Smooth Muscle(SM) protein. Standards or samples are added to the appropriate microtiter plate wells that with Smooth Muscle(SM) protein. Then add a biotin-conjugated Smooth Muscle(SM) protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody(ASMA) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Smooth Muscle Antibody(ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction

Alternative Names:
ASM Ab, Anti Smooth Muscle Antibody

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 500 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only