Pig BALP (Bone-specific alkaline phosphatase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BALP(Bone-specific alkaline phosphatase). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BALP(Bone-specific alkaline phosphatase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BALP(Bone-specific alkaline phosphatase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BALP(Bone-specific alkaline phosphatase) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway, Hepatology, Bone metabolism

Alternative Names:
BALP, BSAP, HOPS, AP-TNAP, TNSALP, Bone Alkaline Phosphatase, Alkaline Phosphatase,Tissue-Nonspecific Isozyme

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 80 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only