Pigeon IgG (Immunoglobulin G) ELISA Kit
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In stock
SKU
ELK9264
Catalog Number: ELK9264
Reactivity: Pigeon
Standard Range: 0.79-50 ug/mL
Sensitivity: 0.38 ug/mL
Information
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Reactivity: Pigeon
Standard Range: 0.79-50 ug/mL
Sensitivity: 0.38 ug/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G(IgG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G(IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G(IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G(IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Infection immunity, Immune molecule, Hematology
Alternative Names:
Immunoglobulin G
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Sandwich
Standard: 50 ug/mL
Assay Length: 3.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G(IgG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G(IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G(IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G(IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Infection immunity, Immune molecule, Hematology
Alternative Names:
Immunoglobulin G
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Sandwich
Standard: 50 ug/mL
Assay Length: 3.5h
Assay Length: For Research Use Only
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