Plant CYT (Cytokinin) ELISA Kit
€0.00
In stock
SKU
ELK8628
Catalog Number: ELK8628
Reactivity: Plant
Standard Range: 0.16-10 ng/mL
Sensitivity: 0.062 ng/mL
Information
Request Manual
Questions? Contact us!
Reactivity: Plant
Standard Range: 0.16-10 ng/mL
Sensitivity: 0.062 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cytokinin(CYT) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokinin(CYT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokinin(CYT) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Enzyme & Kinase
Alternative Names:
CYT
Sample Type:
plant tissues
Assay Type: Competitive Inhibition
Standard: 10 ng/mL
Assay Length: 2h
Assay Length: For Research Use Only
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cytokinin(CYT) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokinin(CYT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokinin(CYT) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Enzyme & Kinase
Alternative Names:
CYT
Sample Type:
plant tissues
Assay Type: Competitive Inhibition
Standard: 10 ng/mL
Assay Length: 2h
Assay Length: For Research Use Only
Is Featured? | No |
---|
Write Your Own Review