Plasma Cell Marker (clone EBS-O-231), anti-human

Source:
A BALB/c mouse was immunized with pancreatic cancer related serum mucin.
Fusion partner: NS1.

Specifications:
EBS-O-231 recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This MAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma.

Species reactivity:
Positive: human. Negative: rat.

Applications:
EBS-O-231 cannot be used for frozen section, it is only suitable for paraffin sections using citrate antigen retrieval step.
Frozen sections: -
Paraffin sections: Citrate

Positive control: Human toncil.

References:
- Turly H., et al. J. Clin. Pathol.39: 418-422 (1994).
- Ching C., et al. Int. J. Cancer 45(6): 1022-7 (1990).
- Ching C., et al. Gastroenetorology95(1): 137-142 (1988)