pMBL™ T Vector

pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase.

These Polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.

Advantages & Features
- Highly efficient: greater than 90% white colonies in a transformation with supplied insert control.
- Proven performance: more than 1,000 recombinant colonies expected in optimal conditions.
- Fast and easy protocol: results from 15 min protocol.
- Optimized: improve efficiency of ligation of a PCR product into the plasmid.
- Compatible: overhang for ligation of PCR products preventing recircularization of the vector.
- Designed by cutting the vector with EcoRV and adding a 3´ terminal thymidine to both ends.

Includes
- 20 µL pMBL™ T-Vector (50 ng/µL)
- 20 µL T4 DNA Ligase (5U/Weiss)
- 100 µL T4 DNA Ligase Buffer (10x)
- 5 µL Insert Control 600 bp (30 ng/µL)

Applications
- Cloning of PCR fragments into DNA Cloning vector.
- Blue/white screening for recombinants.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).