Poly A Polymerase

Poly(A) Polymerase I from Escherichia coli, is a very active recombinant enzyme produced by a overproducer strain of E. coli. The enzyme adds a long tail of adenine nucleotides at the 3’ end of messenger RNA using ATP as donor.

GMP-grade is also available.

Advantages & Features
- High-Purity: ≥ 95% determined by SDS.
- Recombinant: isolated from E. coli.
- Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
- GMP-grade available.

Specifications
Protein information:
- Predicted MW: 54 kDa
- Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
- Buffer Storage: 1X Reaction Buffer: 20 mM Tris-HCl, 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1 % Triton®X-100, 50% Glycerol, (pH 7.5 at 25°C).
- Biological activity: One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37°C.

Gene information:
- Synonyms: PAP I
- Official Symbol: pcnB
- Specie: Escherichia coli
- GenBank accession number: NC_000913 (Range 157729..159126)
- NCBI Protein RefSeq: NP_414685.4
- Protein Family: polyA polymerase

Includes
- 100 U Poly(A) Polymerase, from E. coli (5 U/µL)
- Adenosine-5′-Triphosphate (ATP)
- Poly(A) Polymerase Reaction Buffer

Applications
- Labeling of RNA with ATP or cordycepin.
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhances translation of RNA transferred into eukaryotic cells.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).