Polyclonal Anti-β-Actin loading control antibody
€0.00
In stock
SKU
BTL1026
Catalog Number: BTL1026
Size: 100 μg
Isotype: Rabbit IgG
Reactivity: human, mouse, rat, monkey, D. melanogaster
Application(s): WB
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Size: 100 μg
Isotype: Rabbit IgG
Reactivity: human, mouse, rat, monkey, D. melanogaster
Application(s): WB
Request Information
Background:
Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found thatapproximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation.Andarginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.1Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.2Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.
Immunogen:
A synthetic peptide (conjugated to KLH) corresponding to N-terminal residues of humanβ-Actin.
Specificity: Detect endogenous level of totalβ-Actin protein.Western Blot (1:2000);
Product Form: Liquid
Storage Instruction:
Store at -20℃ for 1 year. Avoid repeated freeze and thaw cycles.
Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found thatapproximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation.Andarginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.1Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.2Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.
Immunogen:
A synthetic peptide (conjugated to KLH) corresponding to N-terminal residues of humanβ-Actin.
Specificity: Detect endogenous level of totalβ-Actin protein.Western Blot (1:2000);
Product Form: Liquid
Storage Instruction:
Store at -20℃ for 1 year. Avoid repeated freeze and thaw cycles.
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