Protein A Green Separopore® 4B Microbeads viewIP™

Application:
The green-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the green beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein A Green-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: Recombinant Protein A from Staphylococcus aureus
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size (range): 52 – 165 µm
Matrix activation: Cyanogen bromide
Binding capacity: ~ 20 mg human IgG/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3-9 (for use), 2-10 (for cleaning)
Supplied as 50% suspension in PBS containing 50% glycerol.
Storage: 2 – 8 °C, For long term, store at -20 °C

References:
Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646. J Bacteriol. (1997) 179: 3482-7.
Purification and characterization of bovine interstitial collagenase and tissue inhibitor of metalloproteinases. Arch Biochem Biophys. (1992) 293: 370-6.

Related products:
View-IP™ Protein A Red-Separopore® 4B-CL (SKU # 20181041)
View-IP™ Protein A Blue-Separopore® 4B-CL (SKU # 20181105)
View-IP™ Protein G Red-Separopore® 4B-CL (SKU # 20181118)
View-IP™ Streptavidin Red-Separopore® 4B-CL (SKU # 20181103)
View-IP™ Streptavidin Green-Separopore® 4B-CL (SKU # 20181109)
View-IP™ Streptavidin Blue-Separopore® 4B-CL (SKU # 20181106)
View-IP™ His-tag Nickel Red-Separopore® 6B-CL (SKU # 20181117)
View-IP™ Glutathione Red-Separopore® 4B-CL (SKU # 20181116)
View-IP™ Calmodulin Red-Separopore® 4B-CL (SKU # 20181104)
View-IP™ Calmodulin Green-Separopore® 4B-CL (SKU # 20181110)
View-IP™ Calmodulin Blue-Separopore® 4B-CL (SKU # 20181107)
View-IP™ Anti-HA Red-Separopore® 4B-CL (SKU # 20181115)
View-IP™ Anti-DYKDDDDK (FLAG) Red-Separopore® 4B-CL (SKU # 20181114)
View-IP™ Anti-cMyc Red-Separopore® 4B-CL (SKU # 20181113)

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™