Protein A Red Separopore® 4B-MB

Application:
Protein-A Red Separopore® 4B-MB functions the same as conventional affinity beads, but have enhanced visibility due to red colored Separopore® macrobeads. The red colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in immunoprecipitation (IP) procedures, thereby increasing efficiency and yield.Affinity matrix with immobilized Protein-A by CNBr activation coupling method.

For immunoprecipitation and co-immunoprecipitation experiments.
Reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: Recombinant Protein-A (42 kDa)
Matrix: Red Separopore® 4B-MB (agarose macrobeads, 4%)
Particle size range: 150 – 265 µm
Matrix activation: Cyanogen bromide
Ligand density: ~ 3 mg Protein A/ml medium
Binding capacity: ~ 20mg human IgG / ml drained gel
Molecular weight range: 6 x 104 – 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C
Supplied as suspension in 0.5M NaCl, 0.02% sodium azide

References:
Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides. (2008) 29: 1271-9.
An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins. Biochim Biophys Acta. (2006) 1764: 1141-6.
Toxicity to cotton boll weevil Anthonomus grandis of a trypsin inhibitor from chickpea seeds. Comp Biochem Physiol B Biochem Mol Biol. (2005) 140: 313-9.
A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides. (2004) 25: 11-7.
Contamination of ribosome inactivating proteins with ribonucleases, separated by affinity chromatography on red sepharose. Prep Biochem Biotechnol. (2003) 33: 101-11.
Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J Biol Chem. (2001) 276: 9059-65.
A carbonyl reductase-catalyzing reduction of N3-phenacyluridine in rabbit liver. Biol Pharm Bull. (2000) 23: 917-21.
Preparation of highly purified momordin II without ribonuclease activity. Life Sci. (1999) 65: 1485-91.
The heparin/heparan sulfate-binding site on apo-serum amyloid A. Implications for the therapeutic intervention of amyloidosis. J Biol Chem. (1999) 274: 7172-81.
Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents. Appl Environ Microbiol. (1998) 64: 2152-7.
Purification and partial characterization of an indomethacin hydrolyzing enzyme from pig liver. Pharm Res. (1996) 13: 1327-35.
Rapid purification of native SecA from Escherichia coli: development of a new affinity chromatography procedure. Curr Microbiol. (1994) 29: 323-9.
Purification and characterization of an autocrine migration factor for vascular smooth muscle cells (SMC), SMC-derived migration factor. J Biol Chem. (1993) 268: 13301-8.
A novel NADPH-dependent cytosolic 3,5,3'-triiodo-L-thyronine-binding protein (CTBP; 5.1S) in rat liver: a comparison with 4.7S NADPH-dependent CTBP. Endocrinology. (1991) 129: 1701-8.
A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies with wheat gamma-purothionins. FEBS Lett. (1991) 279: 101-4.
Purification of cytosolic 3,5,3'-triiodo-L-thyronine(T3)-binding protein(CTBP) which regulates nuclear T3 translocation. Biochem Biophys Res Commun. (1991) 174: 1084-9.
Purification and characterization of rho-crystallin from Japanese common bullfrog lens. J Biol Chem. (1990) 265: 9914-23.
Purification and characterization of an insect alpha-amylase inhibitor/endochitinase from seeds of Job's Tears (Coix lachryma-jobi). Biochim Biophys Acta. (1989) 999: 260-6.
IL-2 receptor(p55)/Tac-inducing factor. Purification and characterization of adult T cell leukemia-derived factor. J Immunol. (1988) 140: 2614-20.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®