Protein A Red Separopore® 4B Microbeads viewIP™
€0.00
In stock
SKU
BW-20181041
Application:
The red-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the red beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein A Red-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Recombinant Protein A from Staphylococcus aureus
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size (range): 52 – 165 µm
Matrix activation: Cyanogen bromide
Binding capacity: ~ 20 mg human IgG/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3-9 (for use), 2-10 (for cleaning)
Supplied as 50% suspension in PBS containing 50% glycerol.
Storage: 2-8 °C, For long term, store at -20 °C
References:
Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides. (2008) 29: 1271-9.
An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins. Biochim Biophys Acta. (2006) 1764: 1141-6.
Toxicity to cotton boll weevil Anthonomus grandis of a trypsin inhibitor from chickpea seeds. Comp Biochem Physiol B Biochem Mol Biol. (2005) 140: 313-9.
A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides. (2004) 25: 11-7.
Contamination of ribosome inactivating proteins with ribonucleases, separated by affinity chromatography on red sepharose. Prep Biochem Biotechnol. (2003) 33: 101-11.
Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J Biol Chem. (2001) 276: 9059-65.
A carbonyl reductase-catalyzing reduction of N3-phenacyluridine in rabbit liver. Biol Pharm Bull. (2000) 23: 917-21.
Preparation of highly purified momordin II without ribonuclease activity. Life Sci. (1999) 65: 1485-91.
The heparin/heparan sulfate-binding site on apo-serum amyloid A. Implications for the therapeutic intervention of amyloidosis. J Biol Chem. (1999) 274: 7172-81.
Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents. Appl Environ Microbiol. (1998) 64: 2152-7.
Purification and partial characterization of an indomethacin hydrolyzing enzyme from pig liver. Pharm Res. (1996) 13: 1327-35.
Rapid purification of native SecA from Escherichia coli: development of a new affinity chromatography procedure. Curr Microbiol. (1994) 29: 323-9.
Purification and characterization of an autocrine migration factor for vascular smooth muscle cells (SMC), SMC-derived migration factor. J Biol Chem. (1993) 268: 13301-8.
A novel NADPH-dependent cytosolic 3,5,3'-triiodo-L-thyronine-binding protein (CTBP; 5.1S) in rat liver: a comparison with 4.7S NADPH-dependent CTBP. Endocrinology. (1991) 129: 1701-8.
A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies with wheat gamma-purothionins. FEBS Lett. (1991) 279: 101-4.
Purification of cytosolic 3,5,3'-triiodo-L-thyronine(T3)-binding protein(CTBP) which regulates nuclear T3 translocation. Biochem Biophys Res Commun. (1991) 174: 1084-9.
Purification and characterization of rho-crystallin from Japanese common bullfrog lens. J Biol Chem. (1990) 265: 9914-23.
Purification and characterization of an insect alpha-amylase inhibitor/endochitinase from seeds of Job's Tears (Coix lachryma-jobi). Biochim Biophys Acta. (1989) 999: 260-6.
IL-2 receptor(p55)/Tac-inducing factor. Purification and characterization of adult T cell leukemia-derived factor. J Immunol. (1988) 140: 2614-20.
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
The red-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the red beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein A Red-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Recombinant Protein A from Staphylococcus aureus
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size (range): 52 – 165 µm
Matrix activation: Cyanogen bromide
Binding capacity: ~ 20 mg human IgG/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3-9 (for use), 2-10 (for cleaning)
Supplied as 50% suspension in PBS containing 50% glycerol.
Storage: 2-8 °C, For long term, store at -20 °C
References:
Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides. (2008) 29: 1271-9.
An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins. Biochim Biophys Acta. (2006) 1764: 1141-6.
Toxicity to cotton boll weevil Anthonomus grandis of a trypsin inhibitor from chickpea seeds. Comp Biochem Physiol B Biochem Mol Biol. (2005) 140: 313-9.
A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides. (2004) 25: 11-7.
Contamination of ribosome inactivating proteins with ribonucleases, separated by affinity chromatography on red sepharose. Prep Biochem Biotechnol. (2003) 33: 101-11.
Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J Biol Chem. (2001) 276: 9059-65.
A carbonyl reductase-catalyzing reduction of N3-phenacyluridine in rabbit liver. Biol Pharm Bull. (2000) 23: 917-21.
Preparation of highly purified momordin II without ribonuclease activity. Life Sci. (1999) 65: 1485-91.
The heparin/heparan sulfate-binding site on apo-serum amyloid A. Implications for the therapeutic intervention of amyloidosis. J Biol Chem. (1999) 274: 7172-81.
Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents. Appl Environ Microbiol. (1998) 64: 2152-7.
Purification and partial characterization of an indomethacin hydrolyzing enzyme from pig liver. Pharm Res. (1996) 13: 1327-35.
Rapid purification of native SecA from Escherichia coli: development of a new affinity chromatography procedure. Curr Microbiol. (1994) 29: 323-9.
Purification and characterization of an autocrine migration factor for vascular smooth muscle cells (SMC), SMC-derived migration factor. J Biol Chem. (1993) 268: 13301-8.
A novel NADPH-dependent cytosolic 3,5,3'-triiodo-L-thyronine-binding protein (CTBP; 5.1S) in rat liver: a comparison with 4.7S NADPH-dependent CTBP. Endocrinology. (1991) 129: 1701-8.
A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies with wheat gamma-purothionins. FEBS Lett. (1991) 279: 101-4.
Purification of cytosolic 3,5,3'-triiodo-L-thyronine(T3)-binding protein(CTBP) which regulates nuclear T3 translocation. Biochem Biophys Res Commun. (1991) 174: 1084-9.
Purification and characterization of rho-crystallin from Japanese common bullfrog lens. J Biol Chem. (1990) 265: 9914-23.
Purification and characterization of an insect alpha-amylase inhibitor/endochitinase from seeds of Job's Tears (Coix lachryma-jobi). Biochim Biophys Acta. (1989) 999: 260-6.
IL-2 receptor(p55)/Tac-inducing factor. Purification and characterization of adult T cell leukemia-derived factor. J Immunol. (1988) 140: 2614-20.
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Calmodulin-Separopore® 4B (SKU # 20181001)
E. coli Protein-Separopore® 4B (SKU # 20181006)
Gelatin-Separopore® 4B (SKU # 20181012)
Heparin-Separopore® 4B (SKU # 20181013)
KLH-Separopore® 4B (SKU # 20181016)
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Protein A-Separopore® 4B-CL (SKU # 20181029)
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Protein G-Separopore® 4B-CL (SKU # 20181030)
Streptavidin-Separopore® 4B (SKU # 20181037)
Ubiquitin-Separopore® 4B (SKU # 20181040)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
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