Protein A Separopore® 4B-CL Kit
€0.00
In stock
SKU
BW-20181101
Application:
IgGPure Protein A Separopore® Kit consists of affinity matrix with immobilized Protein-A (from Staphylococcus aureus) for purification and/or fractionation of IgG subclasses, fractionation of IgG fragments, fractionation of cells tagged with IgG, and isolation of antigen-antibody complexes. In addition, Protein A interacts with some classes of IgM and IgA. IgGPure Protein A-Separopore® kit is reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Protein A Agarose can bind >18 mg of human IgG/ml of drained gel.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Kit contents:
Protein-A Separopore® 4B-CL, 5 x 1 ml column
Binding/Wash Buffer: 100 mL (10X)
IgG Elution buffer: 50 ml (10X)
Neutralization buffer: 20 ml
References:
Immunological studies on glycated human IgG. Life Sci. (2012) 90: 980-7.
Purification and partial characterisation of pectin methylesterase produced by Fusarium asiaticum. Fungal Biol. (2011) 115: 1112-21.
Echinococcus multilocularis: purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection. Exp Parasitol. (2011) 128: 50-6.
Effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy. J Clin Apher. (2010) 25: 315-22.
Dual agarose magnetic (DAM) ChIP. BMC Res Notes. (2009) 2: 250.
Improved secretion of human Fas ligand extracellular domain by N-terminal part truncation in Pichia pastoris and preparation of the N-linked carbohydrate chain trimmed derivative. Protein Expr Purif. (2008) 60: 205-13.
Canine heterophilic antibodies as a source of false-positive B-type natriuretic peptide sandwich ELISA results. Vet Clin Pathol. (2008) 37: 86-95.
An increased level of the Concanavalin A-positive IgG in the serum of patients with gastric cancer as evaluated by a lectin enzyme-linked immunosorbent assay (LELISA). Neoplasma. (2008) 55: 143-50.
Annexins are candidate oviductal receptors for bovine sperm surface proteins and thus may serve to hold bovine sperm in the oviductal reservoir. Biol Reprod. (2007) 77: 906-13.
Treatment of chronic dilated cardiomyopathy with immunoadsorption using the staphylococcal A-agarose column: a comparison of immunoglobulin reduction using two different techniques. J Clin Apher. (2007) 22: 224-32.
Biochemical characterization of an aspartic protease from Vigna radiata: Kinetic interactions with the classical inhibitor pepstatin implicating a tight binding mechanism. Biochim Biophys Acta. (2007) 1774: 619-27.
Binding characteristics of SLE anti-DNA autoantibodies to Catecholestrogen-modified DNA. Scand J Immunol. (2006) 64: 677-83.
Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum. J Cell Physiol. (2006) 209: 353-62.
Glutamic acid reverses Pb2+-induced reductions of NMDA receptor subunits in vitro. Neurotoxicology. (2006) 27: 169-75.
Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR. J Virol Methods. (2005) 128: 183-8.
Thiophilic paramagnetic particles as a batch separation medium for the purification of antibodies from various source materials. Anal Biochem. (2005) 338: 186-91.
Specific detection of cytopathogenic Listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis. J Microbiol Methods. (2005) 60: 259-68.
Analysis of lectin-bound glycoproteins in snake venom from the Elapidae and Viperidae families. J Proteome Res. (2004) 3: 383-92.
Purification of antibodies against N-homocysteinylated proteins by affinity chromatography on Nomega-homocysteinyl-aminohexyl-Agarose. J Chromatogr B Analyt Technol Biomed Life Sci. (2004) 807: 257-61.
Elimination of alkaloids from plant-derived human monoclonal antibody. J Immunol Methods. (2004) 286: 79-85.
Effect of bronchoalveolar lavage fluid from Pneumocystis carinii-infected hosts on phagocytic activity of alveolar macrophages. Infect Immun. (2004) 72: 2140-7.
Effect of the water-soluble and non-dialyzable fraction isolated from Senso (Chan Su) on lymphocyte proliferation and natural killer activity in C3H mice. Biol Pharm Bull. (2004) 27: 256-60.
Aberrant profiles of native and oxidized glycoproteins in Alzheimer plasma. Proteomics. (2003) 3: 2240-8.
Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein. Cell Mol Biol Lett. (2003) 8: 783-92.
Protein disulphide isomerase of Ostertagia ostertagi: an excretory-secretory product of L4 and adult worms? Int J Parasitol. (2003) 33: 129-36.
Characterization of three chitosanase isozymes isolated from a commercial crude porcine pepsin preparation. J Agric Food Chem. (2003) 51: 1042-8.
Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase: a step toward carbon dioxide fixation bioprocess. Biotechnol Bioeng. (2003) 81: 705-11.
The peak height ratio of S-sulfonated transthyretin and other oxidized isoforms as a marker for molybdenum cofactor deficiency, measured by electrospray ionization mass spectrometry. Biochim Biophys Acta. (2002) 1588: 135-8.
Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion. Vet Immunol Immunopathol. (2002) 89: 187-95.
Antigenic characterization of a marine fish iridovirus from grouper, Epinephelus spp. J Virol Methods. (2002) 106: 89-96.
Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress. Indian J Exp Biol. (2001) 39: 786-92.
Separation of an isoenzyme of polyphenol oxidase from Duranta plumieri by expanded bed chromatography. Protein Expr Purif. (2002) 24: 181-7.
Endosomal proteolysis of internalized insulin at the C-terminal region of the B chain by cathepsin D. J Biol Chem. (2002) 277: 9437-46.
Production of a polyclonal antibody against recombinant goldfish prolactin and demonstration of its usefulness in a non-competitive antigen-capture ELISA. Comp Biochem Physiol B Biochem Mol Biol. (2002) 131: 37-46.
Purification and characterization of an extracellular beta-xylosidase from a newly isolated Fusarium verticillioides. J Ind Microbiol Biotechnol. (2001) 27: 241-5.
Glycome project: concept, strategy and preliminary application to Caenorhabditis elegans. Proteomics. (2001) 1: 295-303.
A simple and quick method for enhanced detection of specific IgE in serum from lymphatic filariasis patients. Acta Trop. (2001) 80: 51-7.
Expression of the melanocortin 5 receptor on rat lymphocytes. Biochem Biophys Res Commun. (2001) 281: 1086-92.
Neutralization of hepatitis A virus (HAV) by an immunoadhesin containing the cysteine-rich region of HAV cellular receptor-1. J Virol. (2001) 75: 717-25.
Purification of alpha-amylase isoenzymes from Scytalidium thermophilum on a fluidized bed of alginate beads followed by concanavalin A-agarose column chromatography. Protein Expr Purif. (2000) 20: 162-8.
A novel high-throughput method for accurate, rapid, and economical measurement of multiple type 1 diabetes autoantibodies. J Immunol Methods. (2000) 244: 91-103.
Purification and characterization of a poly(A)-binding protein from chickpea (Cicer arietinum) epicotyl. Indian J Biochem Biophys. (2000) 37: 107-13.
Single-chain antibodies against human insulin-like growth factor I receptor: expression, purification, and effect on tumor growth. Cancer Immunol Immunother. (2000) 49: 243-52.
Purification and characterization of a novel alpha-L-arabinofuranosidase from Pichia capsulata X91. Biosci Biotechnol Biochem. (2000) 64: 1181-8.
Serum estradiol-binding profiles in postmenopausal women undergoing three common estrogen replacement therapies: associations with sex hormone-binding globulin, estradiol, and estrone levels. Menopause. (2000) 7: 243-50.
Related products:
IgG-Separopore® (Agarose)-Fusion kit (SKU # 20181014)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name: bioPLUS™, Separopore®
IgGPure Protein A Separopore® Kit consists of affinity matrix with immobilized Protein-A (from Staphylococcus aureus) for purification and/or fractionation of IgG subclasses, fractionation of IgG fragments, fractionation of cells tagged with IgG, and isolation of antigen-antibody complexes. In addition, Protein A interacts with some classes of IgM and IgA. IgGPure Protein A-Separopore® kit is reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Protein A Agarose can bind >18 mg of human IgG/ml of drained gel.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Kit contents:
Protein-A Separopore® 4B-CL, 5 x 1 ml column
Binding/Wash Buffer: 100 mL (10X)
IgG Elution buffer: 50 ml (10X)
Neutralization buffer: 20 ml
References:
Immunological studies on glycated human IgG. Life Sci. (2012) 90: 980-7.
Purification and partial characterisation of pectin methylesterase produced by Fusarium asiaticum. Fungal Biol. (2011) 115: 1112-21.
Echinococcus multilocularis: purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection. Exp Parasitol. (2011) 128: 50-6.
Effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy. J Clin Apher. (2010) 25: 315-22.
Dual agarose magnetic (DAM) ChIP. BMC Res Notes. (2009) 2: 250.
Improved secretion of human Fas ligand extracellular domain by N-terminal part truncation in Pichia pastoris and preparation of the N-linked carbohydrate chain trimmed derivative. Protein Expr Purif. (2008) 60: 205-13.
Canine heterophilic antibodies as a source of false-positive B-type natriuretic peptide sandwich ELISA results. Vet Clin Pathol. (2008) 37: 86-95.
An increased level of the Concanavalin A-positive IgG in the serum of patients with gastric cancer as evaluated by a lectin enzyme-linked immunosorbent assay (LELISA). Neoplasma. (2008) 55: 143-50.
Annexins are candidate oviductal receptors for bovine sperm surface proteins and thus may serve to hold bovine sperm in the oviductal reservoir. Biol Reprod. (2007) 77: 906-13.
Treatment of chronic dilated cardiomyopathy with immunoadsorption using the staphylococcal A-agarose column: a comparison of immunoglobulin reduction using two different techniques. J Clin Apher. (2007) 22: 224-32.
Biochemical characterization of an aspartic protease from Vigna radiata: Kinetic interactions with the classical inhibitor pepstatin implicating a tight binding mechanism. Biochim Biophys Acta. (2007) 1774: 619-27.
Binding characteristics of SLE anti-DNA autoantibodies to Catecholestrogen-modified DNA. Scand J Immunol. (2006) 64: 677-83.
Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum. J Cell Physiol. (2006) 209: 353-62.
Glutamic acid reverses Pb2+-induced reductions of NMDA receptor subunits in vitro. Neurotoxicology. (2006) 27: 169-75.
Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR. J Virol Methods. (2005) 128: 183-8.
Thiophilic paramagnetic particles as a batch separation medium for the purification of antibodies from various source materials. Anal Biochem. (2005) 338: 186-91.
Specific detection of cytopathogenic Listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis. J Microbiol Methods. (2005) 60: 259-68.
Analysis of lectin-bound glycoproteins in snake venom from the Elapidae and Viperidae families. J Proteome Res. (2004) 3: 383-92.
Purification of antibodies against N-homocysteinylated proteins by affinity chromatography on Nomega-homocysteinyl-aminohexyl-Agarose. J Chromatogr B Analyt Technol Biomed Life Sci. (2004) 807: 257-61.
Elimination of alkaloids from plant-derived human monoclonal antibody. J Immunol Methods. (2004) 286: 79-85.
Effect of bronchoalveolar lavage fluid from Pneumocystis carinii-infected hosts on phagocytic activity of alveolar macrophages. Infect Immun. (2004) 72: 2140-7.
Effect of the water-soluble and non-dialyzable fraction isolated from Senso (Chan Su) on lymphocyte proliferation and natural killer activity in C3H mice. Biol Pharm Bull. (2004) 27: 256-60.
Aberrant profiles of native and oxidized glycoproteins in Alzheimer plasma. Proteomics. (2003) 3: 2240-8.
Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein. Cell Mol Biol Lett. (2003) 8: 783-92.
Protein disulphide isomerase of Ostertagia ostertagi: an excretory-secretory product of L4 and adult worms? Int J Parasitol. (2003) 33: 129-36.
Characterization of three chitosanase isozymes isolated from a commercial crude porcine pepsin preparation. J Agric Food Chem. (2003) 51: 1042-8.
Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase: a step toward carbon dioxide fixation bioprocess. Biotechnol Bioeng. (2003) 81: 705-11.
The peak height ratio of S-sulfonated transthyretin and other oxidized isoforms as a marker for molybdenum cofactor deficiency, measured by electrospray ionization mass spectrometry. Biochim Biophys Acta. (2002) 1588: 135-8.
Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion. Vet Immunol Immunopathol. (2002) 89: 187-95.
Antigenic characterization of a marine fish iridovirus from grouper, Epinephelus spp. J Virol Methods. (2002) 106: 89-96.
Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress. Indian J Exp Biol. (2001) 39: 786-92.
Separation of an isoenzyme of polyphenol oxidase from Duranta plumieri by expanded bed chromatography. Protein Expr Purif. (2002) 24: 181-7.
Endosomal proteolysis of internalized insulin at the C-terminal region of the B chain by cathepsin D. J Biol Chem. (2002) 277: 9437-46.
Production of a polyclonal antibody against recombinant goldfish prolactin and demonstration of its usefulness in a non-competitive antigen-capture ELISA. Comp Biochem Physiol B Biochem Mol Biol. (2002) 131: 37-46.
Purification and characterization of an extracellular beta-xylosidase from a newly isolated Fusarium verticillioides. J Ind Microbiol Biotechnol. (2001) 27: 241-5.
Glycome project: concept, strategy and preliminary application to Caenorhabditis elegans. Proteomics. (2001) 1: 295-303.
A simple and quick method for enhanced detection of specific IgE in serum from lymphatic filariasis patients. Acta Trop. (2001) 80: 51-7.
Expression of the melanocortin 5 receptor on rat lymphocytes. Biochem Biophys Res Commun. (2001) 281: 1086-92.
Neutralization of hepatitis A virus (HAV) by an immunoadhesin containing the cysteine-rich region of HAV cellular receptor-1. J Virol. (2001) 75: 717-25.
Purification of alpha-amylase isoenzymes from Scytalidium thermophilum on a fluidized bed of alginate beads followed by concanavalin A-agarose column chromatography. Protein Expr Purif. (2000) 20: 162-8.
A novel high-throughput method for accurate, rapid, and economical measurement of multiple type 1 diabetes autoantibodies. J Immunol Methods. (2000) 244: 91-103.
Purification and characterization of a poly(A)-binding protein from chickpea (Cicer arietinum) epicotyl. Indian J Biochem Biophys. (2000) 37: 107-13.
Single-chain antibodies against human insulin-like growth factor I receptor: expression, purification, and effect on tumor growth. Cancer Immunol Immunother. (2000) 49: 243-52.
Purification and characterization of a novel alpha-L-arabinofuranosidase from Pichia capsulata X91. Biosci Biotechnol Biochem. (2000) 64: 1181-8.
Serum estradiol-binding profiles in postmenopausal women undergoing three common estrogen replacement therapies: associations with sex hormone-binding globulin, estradiol, and estrone levels. Menopause. (2000) 7: 243-50.
Related products:
IgG-Separopore® (Agarose)-Fusion kit (SKU # 20181014)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name: bioPLUS™, Separopore®
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