Protein A Separopore® 4B Microbeads SuperTrap™ Cartridge

Application:
Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A-conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. bioWORLD's SuperTrap™ Protein A Separopore® Microbeads ofer a high efficiency matrix for for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgGs. This matrix is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: recombinant Protein A from E. coli
Matrix: Separopore® 4B (4%)
Particle size range: 30-50 µm
Matrix activation: Cyanogen bromide
Molecular weight range: 6 x 104 - 2 x 107
Ligand density: ~4 mg Protein A / ml drained gel
Binding capacity: ~ 20 mg human IgG / ml drained gel
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C.
Supplied as 50% slurry in PBS with 0.02% thimerosal

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®