Protein G Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181030
Application:
Protein G binds specifically to Fc regions of many mammalian immunoglobulins.
Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids.
These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments.
Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method.
Protein G-Separopore® 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Native Protein-G from Streptococcus sp.
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Matrix activation: Cyanogen bromide
Particle size range: 53 – 180 µm
Exclusion limit range: 6 x 104 - 2 x 107
Ligand density ~ 2 mg Protein-G / mL drained medium
Binding capacity: ~ 20 mg human IgG / mL drained gel
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C
Supplied as suspension in 20% ethanol
References:
Anti-histone H1 IgGs from blood serum of systemic lupus erythematosus patients are capable of hydrolyzing histone H1 and myelin basic protein. J Mol Recognit. (2010) 23: 495-502.
Strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum. J Pharm Biomed Anal. (2011) 55: 1265-74.
Comparison of methods for depletion of albumin and IgG from equine serum. Vet Clin Pathol. (2010) 39: 337-45.
Generation of a novel high-affinity monoclonal antibody with conformational recognition epitope on human IgM. Appl Biochem Biotechnol. (2010) 162: 1249-57.
Immunoglobulins from scleroderma patients inhibit the muscarinic receptor activation in internal anal sphincter smooth muscle cells. Am J Physiol Gastrointest Liver Physiol. (2009) 297: G1206-13.
An IgG complexed form of vitamin B12 is a common cause of elevated serum concentrations. Clin Biochem. (2010) 43: 82-8.
Characterisation of viral proteins that inhibit Toll-like receptor signal transduction. Methods Mol Biol. (2009) 517: 217-35.
Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P bodies. Nat Protoc. (2009) 4: 674-85.
Complement-mediated bactericidal effect of antibodies in human intravenous preparation against multi-drug resistant Pseudomonas aeruginosa. Jpn J Antibiot. (2008) 61: 379-87.
Characterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients. Clin Endocrinol (Oxf). (2009) 70: 599-605.
Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies. Malar J. (2008) 7: 129.
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect Immun. (2008) 76: 3530-8.
Heparin chromatography to deplete high-abundance proteins for serum proteomics. Clin Chim Acta. (2008) 388: 173-8.
Evaluation of a novel agarose-based synthetic ligand adsorbent for the recovery of antibodies from ovine serum. J Chromatogr B Analyt Technol Biomed Life Sci. (2007) 860: 209-17.
Autoantibodies from Sjögren's syndrome trigger apoptosis in salivary gland cell line. Ann N Y Acad Sci. (2007) 1108: 418-25.
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Appearance Form: Suspension
Appearance Color: White
Brand Name: bioPLUS™, Separopore®
Protein G binds specifically to Fc regions of many mammalian immunoglobulins.
Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids.
These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments.
Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method.
Protein G-Separopore® 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Native Protein-G from Streptococcus sp.
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Matrix activation: Cyanogen bromide
Particle size range: 53 – 180 µm
Exclusion limit range: 6 x 104 - 2 x 107
Ligand density ~ 2 mg Protein-G / mL drained medium
Binding capacity: ~ 20 mg human IgG / mL drained gel
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C
Supplied as suspension in 20% ethanol
References:
Anti-histone H1 IgGs from blood serum of systemic lupus erythematosus patients are capable of hydrolyzing histone H1 and myelin basic protein. J Mol Recognit. (2010) 23: 495-502.
Strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum. J Pharm Biomed Anal. (2011) 55: 1265-74.
Comparison of methods for depletion of albumin and IgG from equine serum. Vet Clin Pathol. (2010) 39: 337-45.
Generation of a novel high-affinity monoclonal antibody with conformational recognition epitope on human IgM. Appl Biochem Biotechnol. (2010) 162: 1249-57.
Immunoglobulins from scleroderma patients inhibit the muscarinic receptor activation in internal anal sphincter smooth muscle cells. Am J Physiol Gastrointest Liver Physiol. (2009) 297: G1206-13.
An IgG complexed form of vitamin B12 is a common cause of elevated serum concentrations. Clin Biochem. (2010) 43: 82-8.
Characterisation of viral proteins that inhibit Toll-like receptor signal transduction. Methods Mol Biol. (2009) 517: 217-35.
Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P bodies. Nat Protoc. (2009) 4: 674-85.
Complement-mediated bactericidal effect of antibodies in human intravenous preparation against multi-drug resistant Pseudomonas aeruginosa. Jpn J Antibiot. (2008) 61: 379-87.
Characterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients. Clin Endocrinol (Oxf). (2009) 70: 599-605.
Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies. Malar J. (2008) 7: 129.
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect Immun. (2008) 76: 3530-8.
Heparin chromatography to deplete high-abundance proteins for serum proteomics. Clin Chim Acta. (2008) 388: 173-8.
Evaluation of a novel agarose-based synthetic ligand adsorbent for the recovery of antibodies from ovine serum. J Chromatogr B Analyt Technol Biomed Life Sci. (2007) 860: 209-17.
Autoantibodies from Sjögren's syndrome trigger apoptosis in salivary gland cell line. Ann N Y Acad Sci. (2007) 1108: 418-25.
Related products:
Avidin-Separopore® 6B (SKU # 20181060)
Calmodulin-Separopore® 4B (SKU # 20181001)
E. coli Protein-Separopore® 4B (SKU # 20181006)
Gelatin-Separopore® 4B (SKU # 20181012)
Heparin-Separopore® 4B (SKU # 20181013)
KLH-Separopore® 4B (SKU # 20181016)
Papain-Separopore® 4B (SKU #20181027)
Protein A-Separopore® 4B-CL (SKU # 20181029)
Protein A-Separopore® 4B-CL (Lyophilized) (SKU # 20181028)
Protein A Blue-Separopore® 4B-MB (Macrobeads) (SKU # 20181042)
Protein A Red-Separopore® 4B-MB (Macrobeads) (SKU # 20181086)
View-IP™ Protein-A Red Separopore® 4B-CL (SKU # 20181041)
Streptavidin-Separopore® 4B (SKU # 20181037)
Ubiquitin-Separopore® 4B (SKU # 20181040)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Appearance Form: Suspension
Appearance Color: White
Brand Name: bioPLUS™, Separopore®
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