Protein G Separopore® 6B-CL

Application:
Protein G binds specifically to Fc regions of many mammalian immunoglobulins.
Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids.
These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments.
Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method.
Protein G-Separopore® 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: Native Protein-G from Streptococcus sp.
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 4%)
Matrix activation: Cyanogen bromide
Particle size range: 52 – 165 µm
Exclusion limit range: 6 x 104 - 2 x 107
Ligand density ~ 2 mg Protein-G / ml drained medium
Binding capacity: ~ 20 mg human IgG / ml drained gel
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C
Supplied as suspension in 0.5M NaCl

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®