Proteinase K, Lyophilized powder

Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. It is a serine protease that exhibits broad cleavage specificity. With a molecular weight 28.900 kD, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids.

It is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).

Advantages & Features
- Highly active and stable: > 30 units/mg protein (hemoglobin, pH 7.5, 37 °C).
- High Quality: free of RNases, DNases and Exonucleases contamination.
- Purified by chromatography.
- Lyophilised format.
- Low cutting specificity.

Specifications
Unit Definition:
Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
- Host: Komagataella phaffii (Pichia pastori).
- Appearance: White lyophilized powder.


Includes
Includes for 30 mg:
- 30 mg Proteinase K (Lyophilized powder, =30 units/mg)

Applications
- Isolation of genomic DNA from cultured cells and tissues.
- Removal of DNases and RNases during DNA and/or RNA purification.
- Determination of enzyme locations.

Shipping & Storage
Shipment: at Ambient Temperature.
Storage: 4 °C or -20 °C.