Proteinase K Solution

Proteinase K Solution is a highly active serine protease that exhibits broad cleavage specificity. Isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. With a molecular weight 28.900 kD, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids.

Advantages & Features
- Highly stable at high temperatures.
- Highly active under denaturing conditions.
- High Quality: free of RNases, DNases and Exonucleases contamination.
- Molecular Biology Grade: proven performance for general Digestion of Proteins.

Specifications
Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%
Unit Definition: Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.

Includes
5 x 1 mL Proteinase K Solution (20 mg/mL). Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%

Applications
- Isolation of genomic DNA from cultured cells and tissues.
- Removal of DNases and RNases during DNA and/or RNA purification.
- Determination of enzyme locations.

Shipping & Storage
Shipment: at Ambient Temperature.
Storage: 4 °C or -20 °C.