pSpark® III

pSpark® III is a highly efficient, accurate and easy-to-use DNA Cloning System that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.

Advantages & Features
- Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
- Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
- Powerful: obtain 5-fold more positive colonies using 10x less DNA insert.
- Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
- High stability: eliminates cloning bias or pitfalls.
- Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
- Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
- High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
- Eliminates positive selection vector.

Includes
- 20 rxn pSpark® III (20 ng/µL)
- 20 µL T4 DNA Ligase (5 Weiss U/µL)
- 100 µL T4 DNA Ligase Buffer (10x)
- 150 µL PEG 6000 (10x)
- 5 µL Insert Control 1 kb (20 ng/µL)

Applications
- Cloning directly from PCR using plasmid cloned genes as template.
- Unpurified PCR cloning.
- Cloning of high fidelity PCR amplified products.
- Production of ssDNA.
- Blue/white screening for recombinants.
- In vitro transcription from T7/SP6 dual-opposed promoters.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).