pSpark® TA Done

pSpark® TA Done is efficient, stable and easy-to-use DNA cloning vector based on an improved TA technology that offers all of the advantages of pSpark® TA with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

Advantages & Features
- Convenient: recognition sites for EcoRI and NotI flanking the insertion site.
- Flexible: allows removing the desired insert DNA with other restriction digestion.
- Efficient: more than 600 white positive colonies expected under optimal conditions.
- Stable: without cloning bias due to transcription of toxic genes.
- Easy-to-use: eliminates screening of recombinants due to its low background (4%).
- Fast protocol: ligation time from 60 minutes to overnight.
- Compatible: with direct cloning of PCR products.
- Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase.
- Cost avoidance: avoids primer phosphorylation.

Includes
- 20 rxn pSpark® TA Done (20ng/µL)
- 20 µL T4 DNA Ligase (5 Weiss U/µL)
- 100 µL T4 DNA Ligase Buffer (10x)
- 5 µL Insert Control 1 kb (20 ng/µL)
- 150 µL PEG 6000 solution (10x)

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).