pSpark® Universal

pSpark® Universal is a highly efficient, accurate and easy-to-use DNA Cloning kit ideal for a broad range of PCR fragments cloning applications. There is a range of DNA Polymerases available that do not generate PCR products with identical ends: proofreading DNA polymerases leave blunt ends while blends of polymerases and non-proofreading DNA polymerases leaves 3´A overhangs. Therefore, it is necessary to employ different vectors to clone both kinds of PCR fragments.

pSpark® Universal DNA cloning kit has been designed to save time, looking for a kit for several cloning scenarios. It is mainly composed of two cloning vectors which allow blunt or TA DNA cloning. For blunt DNA cloning and TA DNA cloning, pSpark® II DNA Cloning vector and pSpark® TA DNA Cloning vector, respectively, are included.

Advantages & Features
- Compatible with Blunt and TA DNA cloning: it is composed by pSpark® II and pSpark® TA DNA Cloning vectors.
- Convenient: ideal for a broad range of PCR fragments cloning applications.
- Versatile: compatible with ny DNA Polymerase.

Includes
'- 20 rxn pSpark® II DNA cloning vector (20ng/µL)
- 20 rxn pSpark® TA DNA cloning vector (50ng/µL)
- 150 µL PEG 6000 solution (10X)
- 5 µL control insert 1kb
- 5 µLcontrol insert (TA) 600kb
- 20 µL T4 DNA ligase (5 Weiss U/µL)
- 100 µLT4 DNA ligase Buffer (10x)

Applications
- Cloning of high fidelity PCR amplified products into pSpark® II Blunt DNA cloning vector.
- Cloning of non-proofreading PCR fragments into pSpark® TA DNA Cloning vector.
- Production of ssDNA.
- In vitro transcription from T7/SP6 dual-opposed promoters.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).