pSpark® V

pSpark® V is a highly efficient, accurate and easy-to-use DNA cloning system developed with low copy number, as a help for cloning of inserts with the highers kb. This low copy variant is also transcription-free, for the most demanding cloning tasks. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region has been truncated).

Advantages & Features
- Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
- Transcription-free.
- Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%).
- High stability: removes cloning bias or pitfalls.
- Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
- Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
- Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
- Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
- Optimized: truncated alpha-peptide coding region.
- Cost avoidance: avoids expensive primer phosphorylation use.
- Eliminates positive selection vector.

Includes
- 20 rxn pSpark® V (20 ng/µL)
- 20 µL T4 DNA Ligase (5 Weiss U/µL)
- 100 µL T4 DNA Ligase Buffer (10x)
- 150 µL PEG 6000 (10x)
- 5 µL Insert Control 1 kb (20 ng/µL)

Applications
- Cloning of toxic genes.
- Cloning of unstable genes, for example genes with repeated sequences.
- Cloning of high fidelity PCR amplified products.
- Production of ssDNA.
- Blue/white screening for recombinants.
- In vitro transcription from T7/SP6 dual-opposed promoters.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).