qMAXSen™ One-Step Green RT-qPCR Kit (Low Rox™)
€0.00
In stock
SKU
E0831
Catalog Number: E0831 (100 rxn), E0833 (500 rxn
For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube
Information
Datasheet
For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube
Information
Datasheet
qMAXSen™ One-Step Green RT-qPCR Kit (Low Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 4x concentration to perform Real-Time PCR using a proprietary green dye. The qPCR MasterMix contains all the reagent (except PCR Primers and Template) needed for running PCR reactions. In addition, a separate RT mix that comprises a balanced mixture of both RTase and RNase Inhibitor is also supplied.
Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.
Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®.
Advantages & Features
- Enhanced specificity, sensitivity, and yields in cDNA Synthesis.
- Versatile: compatible with most used real-time PCR instruments.
- Convenient: includes internal passive reference dye.
- Easy: reaction assembly.
Includes
Includes for 500 rxn (of 20 µl):
- 5 x 500 µL qMAXSen™ One-Step RT-qPCR (4x)
- 5 x 100 μL RT mix
- 5 x 1 mL RNase-free Water
Applications
- Detection and Quantification of DNA and cDNA targets.
- Gene Expression.
- Standard and fast qPCR platforms.
- High Throughput Applications.
Shipping & Storage
Shipment: at Gel Pack.
Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.
Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.
Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®.
Advantages & Features
- Enhanced specificity, sensitivity, and yields in cDNA Synthesis.
- Versatile: compatible with most used real-time PCR instruments.
- Convenient: includes internal passive reference dye.
- Easy: reaction assembly.
Includes
Includes for 500 rxn (of 20 µl):
- 5 x 500 µL qMAXSen™ One-Step RT-qPCR (4x)
- 5 x 100 μL RT mix
- 5 x 1 mL RNase-free Water
Applications
- Detection and Quantification of DNA and cDNA targets.
- Gene Expression.
- Standard and fast qPCR platforms.
- High Throughput Applications.
Shipping & Storage
Shipment: at Gel Pack.
Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.
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