Rabbit Anti-C-Myc Red Separopore® 4B Microbeads viewIP™

Rabbit Anti-C-Myc Red Separopore® 4B Microbeads viewIP™

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SKU
BW-20181113
Catalog Number: BW-20181113
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viewIP™ Anti-c-Myc Red Separopore® 4B-CL Antibody (Polyclonal IgG) (Agarose)

Cat# 20181113
Details
Host: Rabbit
Isotype: IgG
Class: Polyclonal
Immunogen: Synthetic peptide corresponding to Human c-Myc protein

Application:
Red-colored affinity matrix consists of affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to red beaded agarose (Separopore®). The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein. Anti-cMyc Red-Separopore® 4B-CL is convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-cMyc Red-Separopore® 4B-CL is reliable tool for affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Red Separopore® Technical Specifications:
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Concentration: 25 mg Red Separopore® per mL of buffer
Particle size range: 52 – 165 µm
Ligand coupling method: CNBr activation
Binding capacity: approximately 50 µg/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol.
Storage: 4°C

References:
Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced high-copy sequences. EMBO J. (2009) 28: 2932-44.
BRI2 inhibits amyloid beta-peptide precursor protein processing by interfering with the docking of secretases to the substrate. J Neurosci. (2008) 28: 8668-76.
Endocytosis of the aspartic acid/glutamic acid transporter Dip5 is triggered by substrate-dependent recruitment of the Rsp5 ubiquitin ligase via the arrestin-like protein Aly2. Mol Cell Biol. (2010) 30: 5598-607.
RACK1 is a novel interaction partner of PTK7 that is required for neural tube closure. Development. (2011) 138: 1321-7.
Scarecrow-like 3 promotes gibberellin signaling by antagonizing master growth repressor DELLA in Arabidopsis. Proc Natl Acad Sci U S A. (2011) 108: 2160-5.
Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions. J Biol Chem. (2012) 287: 3282-91.
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. (1985) 5: 3610-6.
The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca2+-ATPases are on opposite sides of the membrane. J Biol Chem. (1992) 267: 9321-5.
Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science. (1995) 268: 100-2.
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits. Mol Biol Cell. (1992) 3: 1279-94.
C-myc oncoprotein in bronchial carcinoma: expression in all major morphological types. Anticancer Res. (1990) 10: 623-8.
Evaluation of the ras and myc oncoproteins in benign gastric lesions. Anticancer Res. (1990) 10: 1127-33.
Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues. Anticancer Res. (1989) 9: 715-21.
Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides. Anticancer Res. (1991) 11: 1433-8.
Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms. J Pathol. (1990) 160: 287-93.
Cellular localisation of c-myc product in human colorectal epithelial neoplasia. J Pathol. (1992) 166: 225-33.
Ultrastructural localization of ras-related proteins using epitope-tagged plasmids. J Histochem Cytochem. (1995) 43: 471-80.
Use of antibody fragments (Fv) in immunocytochemistry. J Histochem Cytochem. (1995) 43: 607-14.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
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