Rabbit Anti-HA Red Separopore® 4B Microbeads viewIP™
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In stock
SKU
BW-20181115
viewIP™ Anti-HA Red Separopore® Antibody (Polyclonal IgG) (Agarose)
Cat# 20181115
Details
Host: Rabbit
Isotype: IgG
Class: Polyclonal
Immunogen: Synthetic peptide corresponding to HA
Application:
Red-colored affinity matrix consists of purified anti-HA rabbit polyclonal antibody (Clone HA-7) covalently attached to agarose beads. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA). Convenient for affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cells; greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-HA Red-Separopore® 4B-CL is reliable tool for affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cells.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Concentration: 25 mg Red Separopore® per mL of buffer
Particle size range: 52 – 165 µm
Ligand coupling method: CNBr activation
Binding capacity: ~400 µg HA-tagged fusion protein/mL drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3-9 (for use), 2-10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol.
Storage: 4°C
References:
BRI2 inhibits amyloid beta-peptide precursor protein processing by interfering with the docking of secretases to the substrate. J Neurosci. (2008) 28: 8668-76.
Mechanism of cell cycle regulation by FIP200 in human breast cancer cells. Cancer Res. (2005) 65: 6676-84.
Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells. Cell Signal. (2010) 22: 1300-7.
Hsp70 interacts with the retroviral restriction factor TRIM5alpha and assists the folding of TRIM5alpha. J Biol Chem. (2010) 285: 7827-37.
Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers. Cancer Res. (2010) 70: 8159-68.
Expression levels of a LAR-like receptor protein tyrosine phosphatase correlate with neuronal branching and arbor density in the medicinal leech. Dev Biol. (2010) 344: 346-57.
mTORC1 directly phosphorylates and regulates human MAF1. Mol Cell Biol. (2010) 30: 3749-57.
The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. Development. (2010) 137: 1799-805.
A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes Dev. (2010) 24: 1939-50.
Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Proc Natl Acad Sci U S A. (2010) 107: 3752-7.
14-3-3 epsilon dynamically interacts with key components of mitogen-activated protein kinase signal module for selective modulation of the TNF-alpha-induced time course-dependent NF-kappaB activity. J Proteome Res. (2010) 9: 3465-78.
Functional reconstitution into liposomes of purified human RhCG ammonia channel. PLoS One. (2010) 5: e8921.
p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. J Biol Chem. (2011) 286: 40044-59.
Hepatitis C virus NS2 protein serves as a scaffold for virus assembly by interacting with both structural and nonstructural proteins. J Virol. (2011) 85: 86-97.
The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol. (2011) 31: 2641-52.
Signal-induced Brd4 release from chromatin is essential for its role transition from chromatin targeting to transcriptional regulation. Nucleic Acids Res. (2011) 39: 9592-604.
Iwr1 protein is important for preinitiation complex formation by all three nuclear RNA polymerases in Saccharomyces cerevisiae. PLoS One. (2011) 6: e20829.
Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes. Biochem J. (2012) 441: 927-36.
The RNA helicase Dhh1p cooperates with Rbp1p to promote porin mRNA decay via its non-conserved C-terminal domain. Nucleic Acids Res. (2012) 40: 1331-44.
Ubiquitin-specific protease 4 inhibits mono-ubiquitination of the master growth factor signaling kinase PDK1. PLoS One. (2012) 7: e31003.
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View-IP™ Protein G Red-Separopore® 4B-CL (SKU # 20181118)
View-IP™ Streptavidin Red-Separopore® 4B-CL (SKU # 20181103)
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
Cat# 20181115
Details
Host: Rabbit
Isotype: IgG
Class: Polyclonal
Immunogen: Synthetic peptide corresponding to HA
Application:
Red-colored affinity matrix consists of purified anti-HA rabbit polyclonal antibody (Clone HA-7) covalently attached to agarose beads. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA). Convenient for affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cells; greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-HA Red-Separopore® 4B-CL is reliable tool for affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cells.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Concentration: 25 mg Red Separopore® per mL of buffer
Particle size range: 52 – 165 µm
Ligand coupling method: CNBr activation
Binding capacity: ~400 µg HA-tagged fusion protein/mL drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3-9 (for use), 2-10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol.
Storage: 4°C
References:
BRI2 inhibits amyloid beta-peptide precursor protein processing by interfering with the docking of secretases to the substrate. J Neurosci. (2008) 28: 8668-76.
Mechanism of cell cycle regulation by FIP200 in human breast cancer cells. Cancer Res. (2005) 65: 6676-84.
Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells. Cell Signal. (2010) 22: 1300-7.
Hsp70 interacts with the retroviral restriction factor TRIM5alpha and assists the folding of TRIM5alpha. J Biol Chem. (2010) 285: 7827-37.
Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers. Cancer Res. (2010) 70: 8159-68.
Expression levels of a LAR-like receptor protein tyrosine phosphatase correlate with neuronal branching and arbor density in the medicinal leech. Dev Biol. (2010) 344: 346-57.
mTORC1 directly phosphorylates and regulates human MAF1. Mol Cell Biol. (2010) 30: 3749-57.
The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. Development. (2010) 137: 1799-805.
A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes Dev. (2010) 24: 1939-50.
Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Proc Natl Acad Sci U S A. (2010) 107: 3752-7.
14-3-3 epsilon dynamically interacts with key components of mitogen-activated protein kinase signal module for selective modulation of the TNF-alpha-induced time course-dependent NF-kappaB activity. J Proteome Res. (2010) 9: 3465-78.
Functional reconstitution into liposomes of purified human RhCG ammonia channel. PLoS One. (2010) 5: e8921.
p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. J Biol Chem. (2011) 286: 40044-59.
Hepatitis C virus NS2 protein serves as a scaffold for virus assembly by interacting with both structural and nonstructural proteins. J Virol. (2011) 85: 86-97.
The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol. (2011) 31: 2641-52.
Signal-induced Brd4 release from chromatin is essential for its role transition from chromatin targeting to transcriptional regulation. Nucleic Acids Res. (2011) 39: 9592-604.
Iwr1 protein is important for preinitiation complex formation by all three nuclear RNA polymerases in Saccharomyces cerevisiae. PLoS One. (2011) 6: e20829.
Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes. Biochem J. (2012) 441: 927-36.
The RNA helicase Dhh1p cooperates with Rbp1p to promote porin mRNA decay via its non-conserved C-terminal domain. Nucleic Acids Res. (2012) 40: 1331-44.
Ubiquitin-specific protease 4 inhibits mono-ubiquitination of the master growth factor signaling kinase PDK1. PLoS One. (2012) 7: e31003.
Related products:
View-IP™ Protein A Red-Separopore® 4B-CL (SKU # 20181041)
View-IP™ Protein A Green-Separopore® 4B-CL (SKU # 20181108)
View-IP™ Protein A Blue-Separopore® 4B-CL (SKU # 20181105)
View-IP™ Protein G Red-Separopore® 4B-CL (SKU # 20181118)
View-IP™ Streptavidin Red-Separopore® 4B-CL (SKU # 20181103)
View-IP™ Streptavidin Green-Separopore® 4B-CL (SKU # 20181109)
View-IP™ Streptavidin Blue-Separopore® 4B-CL (SKU # 20181106)
View-IP™ His-tag Nickel Red-Separopore® 6B-CL (SKU # 20181117)
View-IP™ Glutathione Red-Separopore® 4B-CL (SKU # 20181116)
View-IP™ Calmodulin Red-Separopore® 4B-CL (SKU # 20181104)
View-IP™ Calmodulin Green-Separopore® 4B-CL (SKU # 20181110)
View-IP™ Calmodulin Blue-Separopore® 4B-CL (SKU # 20181107)
View-IP™ Anti-DYKDDDDK (FLAG) Red-Separopore® 4B-CL (SKU # 20181114)
View-IP™ Anti-cMyc Red-Separopore® 4B-CL (SKU # 20181113)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
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