Rabbit IgG Separopore® 4B
€0.00
In stock
SKU
BW-20181015
Application:
Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems. IgG (Rabbit)-Separopore® 4B is suitable for antibody purification or to remove unwanted cross-reactivity.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Rabbit IgG
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size: 52 – 165 µm
Matrix activation: Cyanogen bromide
Ligand density: ≥5 mg rabbit IgG / ml drained gel
pH stability: 3 – 9
Storage: 2 – 8 °C
References:
Behaviour of a recombinant cabbage (Brassica oleracea) phospholipase D immobilized on CNBr-activated and antibody supports. Biotechnol Appl Biochem. (2004) 40: 95-9.
Preparation of a highly stable, very active and high-yield multilayered assembly of glucose oxidase using carbohydrate-specific polyclonal antibodies. Biotechnol Appl Biochem. (2004) 39: 233-9.
Immobilization of Saccharomyces cerevisiae cells to protein G-Sepharose by cell wall engineering. J Mol Microbiol Biotechnol. (2003) 5: 161-6.
Human Goodpasture anti-alpha3(IV)NC1 autoantibodies share structural determinants. Kidney Int. (1998) 53: 402-7.
Partially purified Toxoplasma gondii antigens by immunoaffinity chromatography. Korean J Parasitol. (1997) 35: 251-8.
Immunochemical properties of a 60 kDa cell surface-associated heat shock-protein (Hsp60) from Helicobacter pylori. FEMS Immunol Med Microbiol. (1996) 16: 163-72.
Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
Characterization of a novel mouse recombinant processing alpha-mannosidase. Glycobiology. (1994) 4: 445-50.
Rapid isolation and biochemical characterization of rat C1 and C1q. Mol Immunol. (1993) 30: 433-40.
Antibodies to the rat substance P receptor: production and characterization. Cell Mol Neurobiol. (1992) 12: 529-45.
Artificial immunoglobulin G-binding protein mimetic to staphylococcal protein A. Its production and application to affinity purification of immunoglobulin G. J Chromatogr. (1992) 597: 277-83.
A recombinant Escherichia coli heat-stable enterotoxin (STa) fusion protein eliciting anti-STa neutralizing antibodies. FEMS Microbiol Lett. (1991) 66: 271-7.
Identification of components of the endoplasmic reticulum and Golgi complex by murine autoreactive monoclonal antibodies. Immunology. (1991) 72: 418-25.
Growth of rat-mouse hybridoma cells in immunosuppressed hamsters. An easy and effective method to prepare monoclonal antibodies from heterohybridoma cell lines. J Immunol Methods. (1990) 130: 25-31.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity. Hybridoma. (1989) 8: 561-7.
A novel brain-specific antigen: a glycoprotein electrophoretically similar to but immunochemically different from type B nucleoside diphosphatase. J Biochem. (1989) 105: 457-60.
Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity. J Clin Invest. (1989) 83: 713-7.
Detection of glycophorin A-like glycoproteins on the surface of cultured human cells. Int J Biochem. (1989) 21: 635-43.
Cross-reaction of a monoclonal antibody to human MHC class II molecules with rabbit B cells. Mol Immunol. (1988) 25: 713-8.
The interaction of IgG subclasses with solubilized Fc receptors of rat basophilic leukemia cells. Mol Immunol. (1988) 25: 599-609.
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Anti-Bovine Albumin (BSA) Separopore® 4B (SKU # 20181059)
Anti-Bovine Albumin (BSA)-Red Separopore® 4B (SKU # 20181089)
Goat (anti-Rabbit) IgG Separopore® 4B (SKU # 20181090)
Goat Anti-Rabbit IgG (H+L) -Red Separopore® 4B (SKU # 20181081)
Goat (anti-Mouse) IgG Separopore® 4B (SKU # 20181091)
Goat Anti-Mouse IgG (Fc)-Red Separopore® 4B (SKU # 20181082)
IgG (Mouse) Separopore® 4B (SKU # 20181078)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems. IgG (Rabbit)-Separopore® 4B is suitable for antibody purification or to remove unwanted cross-reactivity.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Rabbit IgG
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size: 52 – 165 µm
Matrix activation: Cyanogen bromide
Ligand density: ≥5 mg rabbit IgG / ml drained gel
pH stability: 3 – 9
Storage: 2 – 8 °C
References:
Behaviour of a recombinant cabbage (Brassica oleracea) phospholipase D immobilized on CNBr-activated and antibody supports. Biotechnol Appl Biochem. (2004) 40: 95-9.
Preparation of a highly stable, very active and high-yield multilayered assembly of glucose oxidase using carbohydrate-specific polyclonal antibodies. Biotechnol Appl Biochem. (2004) 39: 233-9.
Immobilization of Saccharomyces cerevisiae cells to protein G-Sepharose by cell wall engineering. J Mol Microbiol Biotechnol. (2003) 5: 161-6.
Human Goodpasture anti-alpha3(IV)NC1 autoantibodies share structural determinants. Kidney Int. (1998) 53: 402-7.
Partially purified Toxoplasma gondii antigens by immunoaffinity chromatography. Korean J Parasitol. (1997) 35: 251-8.
Immunochemical properties of a 60 kDa cell surface-associated heat shock-protein (Hsp60) from Helicobacter pylori. FEMS Immunol Med Microbiol. (1996) 16: 163-72.
Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
Characterization of a novel mouse recombinant processing alpha-mannosidase. Glycobiology. (1994) 4: 445-50.
Rapid isolation and biochemical characterization of rat C1 and C1q. Mol Immunol. (1993) 30: 433-40.
Antibodies to the rat substance P receptor: production and characterization. Cell Mol Neurobiol. (1992) 12: 529-45.
Artificial immunoglobulin G-binding protein mimetic to staphylococcal protein A. Its production and application to affinity purification of immunoglobulin G. J Chromatogr. (1992) 597: 277-83.
A recombinant Escherichia coli heat-stable enterotoxin (STa) fusion protein eliciting anti-STa neutralizing antibodies. FEMS Microbiol Lett. (1991) 66: 271-7.
Identification of components of the endoplasmic reticulum and Golgi complex by murine autoreactive monoclonal antibodies. Immunology. (1991) 72: 418-25.
Growth of rat-mouse hybridoma cells in immunosuppressed hamsters. An easy and effective method to prepare monoclonal antibodies from heterohybridoma cell lines. J Immunol Methods. (1990) 130: 25-31.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity. Hybridoma. (1989) 8: 561-7.
A novel brain-specific antigen: a glycoprotein electrophoretically similar to but immunochemically different from type B nucleoside diphosphatase. J Biochem. (1989) 105: 457-60.
Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity. J Clin Invest. (1989) 83: 713-7.
Detection of glycophorin A-like glycoproteins on the surface of cultured human cells. Int J Biochem. (1989) 21: 635-43.
Cross-reaction of a monoclonal antibody to human MHC class II molecules with rabbit B cells. Mol Immunol. (1988) 25: 713-8.
The interaction of IgG subclasses with solubilized Fc receptors of rat basophilic leukemia cells. Mol Immunol. (1988) 25: 599-609.
Related products:
Anti-Bovine Albumin (BSA) Separopore® 4B (SKU # 20181059)
Anti-Bovine Albumin (BSA)-Red Separopore® 4B (SKU # 20181089)
Goat (anti-Rabbit) IgG Separopore® 4B (SKU # 20181090)
Goat Anti-Rabbit IgG (H+L) -Red Separopore® 4B (SKU # 20181081)
Goat (anti-Mouse) IgG Separopore® 4B (SKU # 20181091)
Goat Anti-Mouse IgG (Fc)-Red Separopore® 4B (SKU # 20181082)
IgG (Mouse) Separopore® 4B (SKU # 20181078)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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