Reactive Blue-4 Separopore® 4B-CL

Application:
Reactive Blue 4-Separopore is a dye affinity matrix used to purify proteins based on selective binding interactions between certain protein sites and the dye molecule. Differerent immobilized dyes bind from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive dyes, such as Reactive Blue 4,to proteins may be due to substrate/cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins. Reactive Blue 4-Separopore® 4B-CL separates proteins based on affinity for this blue dye.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: Reactive Blue 4 dye
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 53 – 180 µm
Matrix activation: Triazine coupling
Ligand density: 2 – 5 mg reactive blue / mL drained gel
Binding capacity: ~5 mg human serum abumin / mL drained gel
pH stability: 3 – 13
Storage: 2 – 8 °C
Supplied as 50% slurry, 0.5M NaCl containing 0.02% sodium azide

References:
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The structure and function of oxidized albumin in hemodialysis patients: Its role in elevated oxidative stress via neutrophil burst. Biochem Biophys Res Commun. (2005) 334: 1322-8.
Purification and characterization of cytosolic pyruvate kinase from developing seeds of Brassica campestris L. Indian J Biochem Biophys. (2000) 37: 51-8.
Suppression of kinetic AMP cooperativity of fructose-1,6-bisphosphatase by carbamoylation of lysine 50. J Protein Chem. (1999) 18: 533-45.
Purification and characterizaton of plastidic pyruvate kinase from developing seeds of Brassica campestris L. Indian J Biochem Biophys. (1998) 35: 346-52.
Latent alpha1-antichymotrypsin. A molecular explanation for the inactivation of alpha1-antichymotrypsin in chronic bronchitis and emphysema. J Biol Chem. (1998) 273: 3695-701.
Purification, characterization, and localization of two ATP diphosphohydrolase isoforms in bovine heart. Am J Physiol. (1997) 273: H673-81.
Triazine dyes inhibit HIV-1 entry by binding to envelope glycoproteins. Microbiol Immunol. (1997) 41: 717-24.
Potentiation of antiproliferative effects of monoclonal antibody Lym-1 and immunoconjugate Lym-1-gelonin on human Burkitt's lymphoma cells with gamma-interferon and tumor necrosis factor. J Immunother Emphasis Tumor Immunol. (1995) 18: 221-30.
A novel method to purify immunotoxins from free antibodies using modified recombinant toxins. J Immunol Methods. (1995) 182: 165-75.
Purification and characterization of the nuclear cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from rat liver. J Biol Chem. (1992) 267: 9257-63.
Dye-affinity purification of transthyretin from an unexploited by-product of human plasma chromatographic fractionation. J Chromatogr. (1992) 576: 87-93.
Purification of rat liver arylsulfatase A and its microheterogeneity assayed by crossed affinity-immunoelectrophoresis. Acta Biochim Pol. (1992) 39: 355-67.
Ovine lactoferrin: isolation from colostrum and characterization. J Dairy Res. (1991) 58: 211-8.
Algal heme oxygenase from Cyanidium caldarium. Partial purification and fractionation into three required protein components. J Biol Chem. (1988) 263: 11915-21.
Interaction of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase with Reactive Blue 2 and related dyes. J Biochem. (1988) 103: 714-21.
Formation of delta-Aminolevulinic Acid from Glutamic Acid in Algal Extracts: Separation into an RNA and Three Required Enzyme Components by Serial Affinity Chromatography. Plant Physiol. (1987) 84: 244-50.
Lung carbonyl reductase in the mouse: biochemical and catalytic properties. Prog Clin Biol Res. (1987) 232: 383-99.
Zonal chromatographic analysis of the interaction of alcohol dehydrogenase with blue-sepharose. J Chromatogr. (1986) 376: 149-55.
Identification of a Mr 40,000 polypeptide from colorectal cancer which expresses organ-specific cancer neoantigen activity as determined by leukocyte adherence inhibition. Cancer Res. (1986) 46: 1874-81.
An isolated enriched organ-specific cancer neoantigen of human lung cancer for leukocyte adherence inhibition assays. Cancer Res. (1985) 45: 2661-9.
Characterization of human S protein, an inhibitor of the membrane attack complex of complement. Demonstration of a free reactive thiol group. Biochemistry. (1985) 24: 2368-74.
The use of thiol-disulphide exchange chromatography for the automated isolation of alpha 1-antitrypsin and other plasma proteins with reactive thiol groups. J Chromatogr. (1983) 278: 53-61.
Hydrophobic interaction chromatography of the rabbit uterine progesterone receptor. J Steroid Biochem. (1983) 19: 1039-45.
Nitro analogs of substrates for adenylosuccinate synthetase and adenylosuccinate lyase. Arch Biochem Biophys. (1983) 225: 157-63.
Subunit structure of rabbit testosterone estradiol-binding globulin. J Biol Chem. (1982) 257: 5118-24.
Isolation of human C-reactive protein and serum amyloid P component. J Immunol Methods. (1982) 50: 17-31.
The purification of glutamine synthetase from Azotobacter and other procaryotes by blue sepharose chromatography. Biochim Biophys Acta. (1979) 568: 428-36.

Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Yellow-Separopore® 4B-CL (SKU # 20181098)
Green-Separopore® 4B-CL (SKU # 20181099)
Brown-Separopore® 4B-CL (SKU # 20181100)

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Appearance Form: suspension
Appearance Color: Blue
Brand Name: bioPLUS™, Separopore®