RNase A

RNase A (Ribonuclease A) is a bovine pancreatic endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5’-ribose of a nucleotide and the phosphate group attached to the 3’-ribose of an adjacent pyrimidine nucleotide. This cleavage forms a 2’, 3’-cyclic phosphate, which is then hydrolysed to the corresponding 3’-nucleoside phosphate.

Advantages & Features
- Small single-chain polypeptide: 124 residues, ~13.7 kDa.
- Avoids the use of co-factors and divalent cations.
- High specific activity: more than 100 Kunitz units per mg protein.
- High Quality: free of DNase, Proteinase or exonuclease contamination.
- Time-saving protocol: avoids the RNAse heat step before use.

Specifications
Unit Definition:
One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm when yeast RNA is hydrolyzed at 37 °C and pH 5.0. 50 units are approximately equivalent to 1 Kunitz unit.

Note: One Kunitz unit equals to 50 Worthington units.

Includes
1 mL RNase A Solution (10 mg/mL)

Applications
- RNA removal during DNA isolation.
- RNA sequence analysis.
- RNase protection assays.
- RNA quantification or mapping.
- Purifying plasmid DNA.
- Genomic DNA isolation.
- Molecular weight marker.

Shipping & Storage
Shipped in: Gel pack.
Storage: RNase A solutions retain enzymatic activity at room temperature or 2-8 ºC for extended periods. However store at –20 ºC in a freezer without a defrost cycle is recommended. Avoid freeze/thaw cycles.