RT-RAA Nucleic Acid Amplification Kit (48)

Intended Purpose
(RT-)RAA Nucleic Acid Amplification Kit (Fluorescent Method) can be used to amplify nucleic acids. The amplification result can be verified by use of fluorescent probes in real-time.

Advantages of Recombinase Aided Amplification (RAA)

Rapid amplification and real-time detection
RAA technology amplifies RNA and DNA in just a few minutes. Positive amplification results are usually detectable after 3 to 10 minutes incubation, provided an appropriate fluorescence reader is used for incubation


Aliquoted freeze-dried reagent mixes
Recombinase Aided Amplification kits are convient to handle. They comprise of freeze-dried enzyme mix aliquoted in standard 0.2mL PCR tubes and of two buffers for rehydration and starting the amplification reaction.

Reverse transcriptase included
In the RT-version of RAA kits, the reverse transcriptase enzyme is included in the freeze-dried reagent pellet. There is no extra pipetting step and no extra costs.

How to use
Protocol for fluorescent RAA assays:
- prepare mastermix of reagents buffer, magnesium acetate and primer-probe mix
- open tube with freeze-dried reagents and add mastermix into the inner surface of the tube lid
- add extracted nucleic acids to the lid as well
- close tube, short-spin and mix by pulse-vortexing
- place tube in your instrument and start incubation and detection (at approx. 37-42°C)

Virus-specific real-time detection assays are separately available for:
- SARS-CoV-2
- Influenza A Virus
- Monkey Pox Virus
- HIV-1
- MERS Coronavirus
- Dengue Virus
- West Nile Virus
- Rift Valley Fever Virus
- Yellow Fever Virus
- Zika Virus
- Chikungunya Virus
- Marburg Virus
- Lassa Fever Virus
- Ebola Virus
- African Swine Fever Virus
- Japanese Encephalitis Virus

Further real-time detection assays available for:
- Mycobacterium tuberculosis
- Vibrio choleraePlasmodium spp.
- Vibrio parahemolyticus
- Schistosoma haematobium
- Salmonella spp.
- Schistosoma mansoni
- Listeria monocytogenes
- Schistosoma japonicum
- Brucella spp.
- Staphylococcus aureus
- Enterobacter sakazakii
- Bacillus anthracis
- Yersinia pestis