SARS-CoV-2 Nucleocapsid antigen-coated beads RUO
€0.00
In stock
SKU
IS-IMS0512
SARS-CoV-2 is a positive-sense, single-stranded RNA virus that has similarities in genome organisation and expression to SARS-CoV, as well as to other human respiratory coronaviruses (NL63, 229E, OC43 and HKU1) and bat coronaviruses, which is its zoonotic reservoir1. From 5’ onwards, two thirds of the SARS-CoV-2 genome encodes for two polyproteins, pp1a and pp1ab, called replicase. These polyproteins are in turn cleaved into 16 non-structural proteins, including RNA-dependent RNA polymerase (RdRp), by the action of two viral proteases essential in virus replication: 3C-type protease (3CLpro) and papain-like protease (PLpro). The other third of the virus genome codes for 8 accessory proteins, which are not essential for replication, and 4 structural proteins, protein S (spicule glycoprotein), protein E (envelope), protein M (membrane) and protein N (nucleocapsid)2 Thus, its genome codes for a total of 28 proteins.
In relation to the viral proteins included in this assay, protein S, which plays a key role in receptor recognition and the cell membrane fusion process, is composed of two subunits, S1 and S2. The S1 subunit contains the receptor-binding domain (RBD) that recognizes and binds to the host receptor’s angiotensin converting enzyme 2 (ECA2), while the S2 subunit facilitates fusion between the viral envelope and the plasma membrane of its target cell. On the other hand, the SARS-CoV-2 viral membrane is surrounded by a helical nucleocapsid in which the viral genome is encapsulated by the nucleocapsid protein (N), this viral protein is produced at high levels within infected cells, improving the efficiency of viral RNA transcription and is essential for viral replication. Lastly, the main viral protease (Mpro or 3CLpro) also plays a critical role in viral replication, which is why it has been proposed as a target for specific inhibitors of virus replication3,4 and has been found to be a potent immunogen
INTENDED USE
SARS-CoV-2 antigen-coated beads are intended for the capture of SARS-COV-2 specific antigenic antibodies and later detection by flow cytometry.
In relation to the viral proteins included in this assay, protein S, which plays a key role in receptor recognition and the cell membrane fusion process, is composed of two subunits, S1 and S2. The S1 subunit contains the receptor-binding domain (RBD) that recognizes and binds to the host receptor’s angiotensin converting enzyme 2 (ECA2), while the S2 subunit facilitates fusion between the viral envelope and the plasma membrane of its target cell. On the other hand, the SARS-CoV-2 viral membrane is surrounded by a helical nucleocapsid in which the viral genome is encapsulated by the nucleocapsid protein (N), this viral protein is produced at high levels within infected cells, improving the efficiency of viral RNA transcription and is essential for viral replication. Lastly, the main viral protease (Mpro or 3CLpro) also plays a critical role in viral replication, which is why it has been proposed as a target for specific inhibitors of virus replication3,4 and has been found to be a potent immunogen
INTENDED USE
SARS-CoV-2 antigen-coated beads are intended for the capture of SARS-COV-2 specific antigenic antibodies and later detection by flow cytometry.
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