SARS-CoV-2 Open Reading Frame-8 (ORF8), Truncated
€350.00
In stock
SKU
IMD-41A255
Origin: Recombinant
Source: E.coli
Tag: None
Purity: >95%
Endotoxin: <5 EU/mg, determined by the LAL method
INTRODUCTION TO THE MOLECULE
The genome of SARS-CoV-2 encodes accessory genes that differ significantly within coronaviruses and contribute to the virus pathogenicity. Among accessory genes, open reading frame 8 (ORF8) stands out by being highly variable and showing structural changes suspected to be related with the virus ability to spread. SARS-CoV-2 ORF8 is a 122 amino acid protein with less than 20% sequence identity to SARS-CoV ORF8. It has an N-terminal signal sequence for the protein secretion, followed by an alpha-helix domain and a beta-sheet containing six strands. The recombinant ORF8 generated by Immunodiagnostics does not contain the a-helix region to increase the solubility.
AMINO ACID SEQUENCE
QHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELC VDEAGSKSPIQYIDIGNYTVSCLP-FTINCQEPKLGSLVVRCSFYEDFLEYHDVRVVLDFI
FORMULATION:Liquid containing ORF8 (2.0 mg/ml) in 50mM Tris, 300mM NaCl, 10% Glycerol, pH8.0.
APPLICATIONS:
ELISA and Western blotting.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
Source: E.coli
Tag: None
Purity: >95%
Endotoxin: <5 EU/mg, determined by the LAL method
INTRODUCTION TO THE MOLECULE
The genome of SARS-CoV-2 encodes accessory genes that differ significantly within coronaviruses and contribute to the virus pathogenicity. Among accessory genes, open reading frame 8 (ORF8) stands out by being highly variable and showing structural changes suspected to be related with the virus ability to spread. SARS-CoV-2 ORF8 is a 122 amino acid protein with less than 20% sequence identity to SARS-CoV ORF8. It has an N-terminal signal sequence for the protein secretion, followed by an alpha-helix domain and a beta-sheet containing six strands. The recombinant ORF8 generated by Immunodiagnostics does not contain the a-helix region to increase the solubility.
AMINO ACID SEQUENCE
QHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELC VDEAGSKSPIQYIDIGNYTVSCLP-FTINCQEPKLGSLVVRCSFYEDFLEYHDVRVVLDFI
FORMULATION:Liquid containing ORF8 (2.0 mg/ml) in 50mM Tris, 300mM NaCl, 10% Glycerol, pH8.0.
APPLICATIONS:
ELISA and Western blotting.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
| Is Featured? | No |
|---|
Write Your Own Review